Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (AB240971)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling NDUFAF5 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (AB240971)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling NDUFAF5 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (AB240971)

Western blot analysis of pellet from 293 cell lysate immunoprecipitated using ab192235 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).

All lanes:

Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (<a href='/ko/products/primary-antibodies/ndufaf5-antibody-epr15870-c-terminal-ab192235'>ab192235</a>)

Predicted band size: 38 kDa

Observed band size: 38 kDa

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• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (AB240971)

Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) cells labelling NDUFAF5 (red) with purified ab192235 at dilution of 1/200. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (AB240971)

Immunofluorescent analysis of NIH 3T3 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NDUFAF5 with ab192235 at 1/400 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls : anti-NDUFAF5 at 1/400 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).