AB110049

Anti-NEIL3 antibody

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Rabbit Polyclonal NEIL3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NEIL3.

대체 명칭 보기

Endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3, NEIL3

1 이미지

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Western blot - Anti-NEIL3 antibody (AB110049)

All lanes:

Western blot - Anti-NEIL3 antibody (ab110049) at 1/500 dilution

Lane 1:

COLO cell extract at 30 µg

Lane 2:

HuvEc cell extract at 30 µg

Lane 3:

HepG2 cell extract at 30 µg

Lane 4:

HeLa cell extract at 30 µg

Lane 5:

HuvEc cell extract at 30 µg with immunising peptide

Predicted band size: 68 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human NEIL3. The exact immunogen used to generate this antibody is proprietary information.

Q8TAT5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NEIL3 also called Nei-like DNA glycosylase 3 plays an important role in DNA repair processes. It is a DNA glycosylase enzyme that weighs approximately 66 kDa. NEIL3 removes oxidized bases from DNA and is expressed in high levels in the thymus testis and brain tissues. The enzyme performs base excision repair which involves recognizing and excising damaged bases thereby maintaining genomic stability.

Biological function summary

NEIL3 participates in the maintenance of an organism's genome integrity. It works as a part of the base excision repair complex interacting with other proteins to repair DNA damage caused by oxidation and alkylation. The enzyme interacts with proliferating cell nuclear antigen (PCNA) to process damage during DNA replication. Its ability to handle a wide range of oxidative lesions is significant for cellular response to DNA damage.

Pathways

NEIL3 is closely integrated into the base excision repair and DNA replication pathways. The base excision repair pathway finds NEIL3 working alongside other DNA glycosylases like OGG1 and NTHL1. It also connects with components like DNA polymerase beta (POLB) for excision and repair. In the DNA replication pathway NEIL3 interacts with DNA replication machinery to maintain replication fork stability collaborating with proteins like RAD51 for homologous recombination and repair processes.

Researchers link NEIL3 to cancer and neurological diseases. Alterations in NEIL3 expression or function often result in genomic instability contributing to oncogenesis. Studies find links between NEIL3 malfunction and certain brain disorders where it may interact with Alzheimer's disease-related proteins like amyloid precursor protein (APP). Targeting NEIL3 pathways could provide therapeutic routes for treating these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA) (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964). Mediates interstrand cross-link repair in response to replication stress : acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases (By similarity). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964). No activity on 8-oxoG detected (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964). Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964). In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues (PubMed : 12433996, PubMed : 19170771, PubMed : 22569481, PubMed : 23755964).

See full target information NEIL3

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 7:43846 PubMed28266613

2017

Screening with an NMNAT2-MSD platform identifies small molecules that modulate NMNAT2 levels in cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yousuf O Ali,Gillian Bradley,Hui-Chen Lu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NEIL3 antibody

Western blot - Anti-NEIL3 antibody (AB110049)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise