AB237027

Anti-NELF-B antibody

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Rabbit Polyclonal NELF-B antibody. Suitable for ChIP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NELFB aa 1-200.

대체 명칭 보기

COBRA1, KIAA1182, NELFB, Negative elongation factor B, NELF-B, Cofactor of BRCA1

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody (AB237027)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody (AB237027)

Paraffin-embedded human colon cancer tissue stained for NELF-B with ab237027 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody (AB237027)

Paraffin-embedded human endometrial cancer tissue stained for NELF-B with ab237027 at 1/100 dilution in immunohistochemical analysis.

ChIP

Supplier Data

ChIP - Anti-NELF-B antibody (AB237027)

HeLa (human epithelial cell line from cervix adenocarcinoma) (1.1 x 10⁶) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 μg ab237027 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers against the TFF1 promoter.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ChIP, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NELFB aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q8WX92

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "Use 4 μg for 1.1 x 106 cells.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NELF-B also known as Negative Elongation Factor B functions mechanically as part of the NELF complex that interacts with RNA polymerase II to pause transcription elongation. NELF-B's mass is about 66 kDa. This protein primarily expresses in the nucleus of cells particularly in tissue types where transcription regulation is essential like neurons and muscle cells which require precise gene expression control.

Biological function summary

NELF-B plays a role in regulating gene expression by pausing RNA polymerase II during early elongation. It operates as part of the larger NELF complex which also includes instigates the pausing mechanism. The NELF complex allows for the fine-tuning of transcription and is important for processes involving cell differentiation and response to external stimuli.

Pathways

NELF-B integrates within transcriptional regulation and RNA processing pathways. It directly affects the transcription cycle by contributing to the controlled release of RNA polymerase II from the paused state. NELF-B relates to proteins like DSIF (DRB Sensitivity-Inducing Factor) which collaborates with the NELF complex to modulate transcription dynamics and ensure proper gene expression regulation.

Aberrant function or expression of NELF-B links to cancer and cardiac hypertrophy. In cancer the dysregulation of transcriptional pausing by NELF-B can lead to unchecked cell proliferation. NELF-B also interacts with proteins like MYC where altered expression can amplify oncogenic pathways. In cardiac hypertrophy improper transcription regulation impacts heart muscle cell function emphasizing NELF-B's role in maintaining cellular homeostasis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed : 12612062). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed : 10199401). May be able to induce chromatin unfolding (PubMed : 11739404). Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes (By similarity). Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules (By similarity). Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes (By similarity).. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II (PubMed : 23884411). In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1 (PubMed : 23884411).

See full target information NELFB

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 30:e13562 PubMed38279604

2024

Cosentyx alleviates psoriasis-induced podocyte injury by inhibiting the tlr/nf-κb signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Aixue,Wei Feng,Zhang Huanhuan,M Xixing,L Yanling

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NELF-B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody (AB237027)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELF-B antibody (AB237027)

ChIP - Anti-NELF-B antibody (AB237027)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise