Anti-NELFB Antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NELFB Antibody (AB327200)

Immunofluorescent staining of human cell line A-431 using ab327200 at 0.25–2 µg/ml, showing localization to nucleoplasm.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELFB Antibody (AB327200)

Immunohistochemical staining of human skin using ab327200 at 1 : 20–1 : 50 dilution, showing moderate nuclear positivity in squamous epithelial cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELFB Antibody (AB327200)

Immunohistochemical staining of human fallopian tube using ab327200 at 1 : 20–1 : 50 dilution, showing moderate nuclear positivity in glandular cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELFB Antibody (AB327200)

Immunohistochemical staining of human testis using ab327200 at 1 : 20–1 : 50 dilution, showing moderate nuclear positivity in cells in seminiferous ducts.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELFB Antibody (AB327200)

Immunohistochemistry analysis in human skin and liver tissues using ab327200 at 1 : 20–1 : 50 dilution. Corresponding NELFB RNA-seq data are presented for the same tissues.

• ChIP

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ChIP - Anti-NELFB Antibody (AB327200)

ChIP-Exo-Seq composite graph for ab327200 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

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Western blot - Anti-NELFB Antibody (AB327200)

Western blot analysis using ab327200 at 0.04–0.4 µg/ml.
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : Human cell line RT-4
Lane 3 : Human cell line U-251MG sp

All lanes:

Western blot - Anti-NELFB Antibody (ab327200)

Lane 2:

Human cell line RT-4

Lane 3:

Human cell line U-251MG sp

Predicted band size: 65.7 kDa

false

• WB

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Western blot - Anti-NELFB Antibody (AB327200)

Western blot analysis using ab327200 at 0.04–0.4 µg/ml.
Lane 1 : NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)

All lanes:

Western blot - Anti-NELFB Antibody (ab327200)

Lane 1:

NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)

Lane 2:

NBT-II cell lysate (Rat Wistar bladder tumour cells)

Predicted band size: 65.7 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELFB Antibody (AB327200)

Immunohistochemical staining of human liver using ab327200 at 1 : 20–1 : 50 dilution, showing no positivity in hepatocytes.