AB327231

Anti-NELFE Antibody

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Rabbit Polyclonal NELFE antibody. Suitable for WB, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NELFE.

대체 명칭 보기

RD, RDBP, NELFE, Negative elongation factor E, NELF-E, RNA-binding protein RD

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-NELFE Antibody (AB327231)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NELFE Antibody (AB327231)

Immunofluorescent staining of human cell line A-431 using ab327231 at 0.25–2 µg/ml, showing localization to nucleoplasm & nuclear bodies.

ChIP

Supplier Data

ChIP - Anti-NELFE Antibody (AB327231)

ChIP-Exo-Seq composite graph for ab327231 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NELFE.

P18615

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed : 10199401, PubMed : 27256882). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed : 11940650, PubMed : 12612062, PubMed : 27256882). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed : 18303858, PubMed : 27256882, PubMed : 27282391).. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.

See full target information NELFE

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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