AB244408

Anti-Neogenin antibody

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Rabbit Polyclonal Neogenin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NEO1 aa 1100 to C-terminus.

대체 명칭 보기

IGDCC2, NGN, NEO1, Neogenin, Immunoglobulin superfamily DCC subclass member 2

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Paraffin-embedded human prostate tissue stained for Neogenin using ab244408 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Paraffin-embedded human kidney tissue stained for Neogenin using ab244408 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Paraffin-embedded human colon tissue stained for Neogenin using ab244408 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody (AB244408)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody (AB244408)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Neogenin (green) using ab244408 at 4 μg/ml in ICC/IF.

Supplier Data

Western blot - Anti-Neogenin antibody (AB244408)

All lanes:

Western blot - Anti-Neogenin antibody (ab244408) at 0.4 µg/mL

Lane 1:

A549 (Human lung carcinoma cell line) cell lysate

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 160 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NEO1 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92859

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Neogenin also known as NEO1 acts as an important receptor involved in cell adhesion migration and neural development. It weighs approximately 186 kDa. Neogenin binds to netrins and repulsive guidance molecules (RGMs) playing a role in the organization of neural circuits. Expressed in tissues such as the developing nervous system muscle tissue and some cancer cells Neogenin collaborates with cellular structures to influence growth and directionality.

Biological function summary

Neogenin influences cell orientation and axon guidance. It forms part of receptor complexes that include proteins like RGM and attracts molecules involved in neural signal transmission. Through these complexes Neogenin impacts processes such as synaptic development and neuronal positioning. Its activity allows for proper nervous system formation and function contributing to precise cellular communication.

Pathways

Neogenin participates in the signaling pathways important for neural pattern formation and tissue structuring. The Netrin signaling pathway is important for neuron navigation where Neogenin interacts with proteins like DCC (Deleted in Colorectal Cancer). Neogenin also engages in the Bone Morphogenetic Protein (BMP) pathways regulating cellular differentiation and tissue architecture.

Neogenin mutations or irregular expressions often associate with neurological disorders and cancer. In particular its dysregulation links to conditions such as brain tumors and congenital brain malformations. In cancer pathways Neogenin connects with proteins like Netrin-1 influencing pathways that lead to tumorigenesis and tumor progression. Understanding Neogenin interactions can help in developing therapeutic strategies targeting these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response.

See full target information NEO1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Extracellular vesicles and circulating nucleic acids 5:397-416 PubMed39697634

2024

Transfer of and increases 3D growth and invasiveness in recipient cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah M Nelson,Shimian Qu,Liyu Huang,Muhammad Shameer,Kevin C Corn,Sydney N Chapman,Nicole L Luthcke,Sara A Schuster,Tellie D Stamaris,Lauren A Turnbull,Lucas L Guy,Xiao Liu,Danielle L Michell,Elizabeth M Semler,Kasey C Vickers,Qi Liu,Jeffrey L Franklin,Alissa M Weaver,Marjan Rafat,Robert J Coffey,James G Patton

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neogenin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neogenin antibody (AB244408)

Immunocytochemistry/ Immunofluorescence - Anti-Neogenin antibody (AB244408)

Western blot - Anti-Neogenin antibody (AB244408)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise