Anti-Nephrin antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody (AB235903)

Immunohistochemical analysis of paraffin-embedded human colorectal cancer with ab235903 diluted at 1 : 100 and performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labelled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody (AB235903)

Immunohistochemical analysis of paraffin-embedded human breast cancer with ab235903 diluted at 1 : 100 and performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labelled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

• WB

Supplier Data

Western blot - Anti-Nephrin antibody (AB235903)

All lanes:

Western blot - Anti-Nephrin antibody (ab235903) at 1/1000 dilution

Lane 1:

JK whole cell lysate at 30 µg

Lane 2:

U251 whole cell lysate at 30 µg

Lane 3:

MCF7 whole cell lysate at 30 µg

Lane 4:

293T whole cell lysate at 30 µg

Observed band size: 135 kDa

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