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AB239476

Anti-Nephrin antibody [C8]

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(1 출판물)

Mouse Monoclonal Nephrin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NPHS1 aa 1150 to C-terminus.

대체 명칭 보기

NPHN, NPHS1, Nephrin, Renal glomerulus-specific cell adhesion receptor

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody [C8] (AB239476)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody [C8] (AB239476)

Paraffin-embedded human kidney tissue stained for Nephrin using ab239476 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-Nephrin antibody [C8] (AB239476)
  • WB

Supplier Data

Western blot - Anti-Nephrin antibody [C8] (AB239476)

All lanes:

Western blot - Anti-Nephrin antibody [C8] (ab239476) at 1 µg/mL

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Predicted band size: 134 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

C8

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NPHS1 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60500

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대체품

Primary Antibodies

AB216341

Anti-Nephrin antibody [EPR20993]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody [EPR20993] (AB216341)

  • 4
  • 1
Primary Antibodies

AB80298

Anti-Nephrin (phospho Y1217) antibody [EPTPG3]

RabMAb|Recombinant

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (AB80298)

  • 0
  • 0
Primary Antibodies

AB80299

Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1]

RabMAb

Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (AB80299)

  • 0
  • 0
Primary Antibodies

AB239895

Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] - BSA and Azide free

RabMAb

Dot Blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] - BSA and Azide free (AB239895)

  • 0
  • 0
Primary Antibodies

AB227806

Anti-Nephrin antibody [EPR20993] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody [EPR20993] - BSA and Azide free (AB227806)

  • 0
  • 0
Primary Antibodies

AB239894

Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (AB239894)

  • 0
  • 0

보완 제품

Assay Kits

AB241018

Albumin Creatinine Ratio Assay Kit

Functional Studies - Albumin Creatinine Ratio Assay Kit (AB241018)

  • 0
  • 0
Primary Antibodies

AB181143

Anti-NPHS2 antibody [EPR13820]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB89901

Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB89901)

  • 5
  • 43
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from TCS.
보관 버퍼
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Nephrin also known as NPHS1 is a transmembrane protein with a molecular weight of approximately 180 kDa. Researchers have predominantly observed nephrin in the podocytes of the kidney glomerulus. Nephrin plays a mechanical role as part of the slit diaphragm a structure critical for maintaining the filtration barrier of the kidney. Its structure includes multiple immunoglobulin-like domains which facilitate cell signaling and adhesion processes important for podocyte function.
Biological function summary

Nephrin is essential for the regulation of glomerular permeability and podocyte foot process architecture. It serves as a significant component of the slit diaphragm complex working alongside proteins like podocin and CD2AP. This protein complex helps regulate the integrity and selectivity of the glomerular filtration barrier ensuring that blood plasma contents are selectively filtered while preventing the loss of large proteins into urine.

Pathways

Nephrin is integral to the PI3K/Akt signaling pathway influencing cell survival and cytoskeletal organization in podocytes. Nephrin's interaction within this pathway involves related proteins such as GAPDH and PTPRO which impact cellular functions critical to maintaining kidney structure and function. Additionally nephrin participates in the synaptopodin pathway modulating actin dynamics and therefore playing a role in cellular contraction and movement.

Nephrin dysfunction is closely related to congenital nephrotic syndrome particularly Finnish-type nephrosis which results in significant proteinuria and kidney failure. Nephrin mutations or aberrant expression can also contribute to other glomerular diseases such as focal segmental glomerulosclerosis (FSGS). In these conditions nephrin's relationship with other proteins like neph1 and podocin becomes disrupted leading to loss of slit diaphragm integrity and impaired renal function.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).
See full target information NPHS1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Respiratory research 24:83 PubMed36927357

2023

Proteome of airway surface liquid and mucus in newborn wildtype and cystic fibrosis piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Ana M Rodriguez-Piñeiro,Florian Jaudas,Nikolai Klymiuk,Andrea Bähr,Gunnar C Hansson,Anna Ermund
제품이 사용된 논문 모두 보기
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Product promise

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