AB239894

Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Nephrin phospho Y1217 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 1 publication.

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Supplier Data

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (AB239894)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80298).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (<a href='/ko/products/primary-antibodies/nephrin-phospho-y1217-antibody-eptpg3-ab80298'>ab80298</a>) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged empty vector whole cell lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) transfected with with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate at 15 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate. Then the membrane was incubated with Alkaline phosphatase at 15 µg

Predicted band size: 134 kDa

Observed band size: 170 kDa

false

Supplier Data

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (AB239894)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Predicted band size: 134 kDa

Observed band size: 170 kDa

false

Supplier Data

Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (AB239894)

Dot blot analysis of Nephrin (pY1217) peptide and Nephrin non-phospho peptide labelling PKC alpha Nephrin (pY1217) with ab80298 at a 1/1000 dilution. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1/20,000 dilution. Blocking and dilution buffer : 5% NFDM/TBST.

Lane 1 : Nephrin (pY1217) phospho peptide.

Lane 2 : Nephrin non-phospho peptide.

Unknown

Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (AB239894)

Dot blot analysis of Nephrin (pY1217) phospho peptide (Lane 1) and Nephrin non-phospho peptide (Lane 2) labelling Nephrin (pY1217) with ab80298 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80298).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPTPG3

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

ab239894 is the carrier-free version of ab80298.

This product has switched from a hybridoma to recombinant production method on 20th November 2023.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Nephrin also known as NPHS1 is a transmembrane protein with a molecular weight of approximately 180 kDa. Researchers have predominantly observed nephrin in the podocytes of the kidney glomerulus. Nephrin plays a mechanical role as part of the slit diaphragm a structure critical for maintaining the filtration barrier of the kidney. Its structure includes multiple immunoglobulin-like domains which facilitate cell signaling and adhesion processes important for podocyte function.

Biological function summary

Nephrin is essential for the regulation of glomerular permeability and podocyte foot process architecture. It serves as a significant component of the slit diaphragm complex working alongside proteins like podocin and CD2AP. This protein complex helps regulate the integrity and selectivity of the glomerular filtration barrier ensuring that blood plasma contents are selectively filtered while preventing the loss of large proteins into urine.

Pathways

Nephrin is integral to the PI3K/Akt signaling pathway influencing cell survival and cytoskeletal organization in podocytes. Nephrin's interaction within this pathway involves related proteins such as GAPDH and PTPRO which impact cellular functions critical to maintaining kidney structure and function. Additionally nephrin participates in the synaptopodin pathway modulating actin dynamics and therefore playing a role in cellular contraction and movement.

Nephrin dysfunction is closely related to congenital nephrotic syndrome particularly Finnish-type nephrosis which results in significant proteinuria and kidney failure. Nephrin mutations or aberrant expression can also contribute to other glomerular diseases such as focal segmental glomerulosclerosis (FSGS). In these conditions nephrin's relationship with other proteins like neph1 and podocin becomes disrupted leading to loss of slit diaphragm integrity and impaired renal function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

See full target information NPHS1 pY1217

대체 명칭 보기

NPHN, NPHS1, Nephrin, Renal glomerulus-specific cell adhesion receptor

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biochemical and biophysical research communications 631:138-145 PubMed36183555

2022

SARS-CoV-2-induced hypomethylation of the ferritin heavy chain (FTH1) gene underlies serum hyperferritinemia in severe COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jibran Sualeh Muhammad,Gehad ElGhazali,Jasmin Shafarin,Mohammad G Mohammad,Ameera Abu-Qiyas,Mawieh Hamad

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (AB239894)

Western blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (AB239894)

Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (AB239894)

Dot Blot - Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (AB239894)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise