AB317024

Anti-Nesprin 2 antibody [EPR28138-90]

BOND RX™ Validated
20ul selling size
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal Nesprin 2 antibody. Suitable for IHC-P, ICC/IF, IHC-Fr and reacts with Mouse samples.

대체 명칭 보기

Nesprin-2, KASH domain-containing protein 2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, Synaptic nuclear envelope protein 2, KASH2, Protein NUANCE, Syne-2

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Nesprin 2 with ab317024 at 1/100 (5.13 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse lung.
The section was incubated with ab317024 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Nesprin 2 with ab317024 at 1/100 (5.13 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse testis.
The section was incubated with ab317024 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis (fresh frozen) tissue labeling Nesprin 2 with ab317024 at 1/1000 (0.513 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Confocal image showing positive staining on mouse testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317024 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling Nesprin 2 with ab317024 at 1/100 (5.13 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse skin.
The section was incubated with ab317024 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized mIMCD3 (mouse inner medullary collecting duct epithelial cell) cells labelling Nesprin 2 with ab317024 at 1/500 (1.026 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing nuclear envelope staining in mIMCD3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression : A20.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28138-90

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, ICC/IF, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Nesprin-2 also known as Nesprin-2 or ABP-Rince2002 is a mechanical protein that plays an important role in the structure of cells. This protein is part of the spectrin repeat-containing nuclear envelope (KASH) proteins with a large mass. It is mainly expressed in tissues such as skeletal muscle the heart and brain. Nesprin-2 is found in the outer nuclear membrane where it functions as a mediator linking the nuclear envelope to the cytoskeleton facilitating mechanical coupling between the nucleus and the cytoplasm.

Biological function summary

Nesprin-2 is involved in various cellular processes that contribute to nuclear positioning and cell movement. It interacts with other nuclear envelope proteins like SUN proteins forming the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex which ensures mechanical communication within cells. This complex supports the anchoring and movement of the nucleus during cytoskeletal changes. Nesprin-2's anchoring abilities make it essential for maintaining cellular integrity and adaptability during mechanical stress.

Pathways

Nesprin-2 participates in mechanotransduction pathways that convert mechanical stimuli into biochemical signals. It plays a critical role in the actin cytoskeleton organization and nuclear positioning pathways. Through these pathways Nesprin-2 interacts with proteins such as F-actin which are important for maintaining cell shape and mobility. The interactions between Nesprin-2 and cytoskeletal elements help facilitate cell signaling in response to mechanical forces.

Nesprin-2 is linked to muscular dystrophies and cardiomyopathies. In muscular dystrophies mutations in Nesprin-2 or its interacting partners like Emerin impair nuclear-cytoskeletal coupling leading to muscle weakness. In cardiomyopathies Nesprin-2 dysfunction affects cardiac muscle integrity and function where it may interact with proteins like Lamin A/C. Understanding these connections offers potential avenues for researching therapeutic interventions for these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probable association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 seem to act redundantly in cerebellum, midbrain, brain stem, and other brain regions except cerebral cortex and hippocampus. During INM at G1 phase mediates respective LINC complex association with kinesin to push the nucleus away from the centrosome. Involved in nuclear migration in retinal photoreceptor progenitors. Required for centrosome migration to the apical cell surface during early ciliogenesis.

See full target information Syne2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nesprin 2 antibody [EPR28138-90]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

Immunohistochemistry (Frozen sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

Immunocytochemistry/ Immunofluorescence - Anti-Nesprin 2 antibody [EPR28138-90] (AB317024)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise