AB192234

Anti-Nesprin1/Syne-1 antibody [EPR14196]

RabMAb
Recombinant
각 태그의 뜻

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(20 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Nesprin1/Syne-1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 20 publications.

대체 명칭 보기

C6orf98, KIAA0796, KIAA1262, KIAA1756, MYNE1, SYNE1, Nesprin-1, Enaptin, KASH domain-containing protein 1, Myocyte nuclear envelope protein 1, Nuclear envelope spectrin repeat protein 1, Synaptic nuclear envelope protein 1, KASH1, Myne-1, Syne-1

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Nesprin 1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Nesprin 1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Nesprin1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

All lanes:

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HACAT cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 1011 kDa

false

Supplier Data

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

All lanes:

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234) at 1/1000 dilution

Lane 1:

RAW 264.7 cell lysate at 10 µg

Lane 2:

PC12 cell lysate at 10 µg

Lane 3:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 1011 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14196

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is not suitable for using in tissue samples when testing in WB application.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Nesprin1 also known as Syne-1 is a large spectrin repeat protein with a mass of approximately 1002 kDa. It is part of the plakin family of proteins and plays an important role in linking the nuclear envelope to the actin cytoskeleton. Nesprin1 is widely expressed in skeletal and cardiac muscle tissues where it helps maintain nuclear positioning and structural integrity. This protein has several isoforms resulting from alternative splicing which allows it to perform diverse functions in different cellular contexts.

Biological function summary

Nesprin1 acts as an important component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. The LINC complex connects the nuclear envelope to the cytoskeleton facilitating mechanical signal transduction and cellular communication. Nesprin1's interactions with other elements of the cytoskeleton and nuclear envelope help regulate nuclear anchoring and positioning essential for cellular dynamics and function. In muscle cells Nesprin1 stabilizes the nucleus and synchronizes cellular responses to mechanical stress.

Pathways

Nesprin1 participates in nuclear-cytoskeletal coupling pathways that are vital for cellular biomechanics. It associates with proteins such as SUN1 and SUN2 within the LINC complex to mediate structural continuity between the nucleus and the cytoskeleton. Furthermore it plays a role in the mechanotransduction pathway linking external mechanical signals to gene expression changes. This connection is significant for maintaining cellular homeostasis and adaptability in response to mechanical stress.

Nesprin1 mutations are associated with muscular dystrophies particularly Emery-Dreifuss muscular dystrophy (EDMD). This condition involves muscle weakness and cardiac issues highlighting the importance of Nesprin1 in muscle tissue integrity. Additionally Nesprin1 has been linked to cardiomyopathies where its interaction with proteins like emerin and lamin A/C is important for maintaining cardiac muscle health. Alterations in these interactions can lead to disrupted nuclear architecture and contribute to the pathogenesis of these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes; SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenesis; a probable SUN3 : SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.

See full target information SYNE1

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제품이 사용된 논문 (20)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature cardiovascular research 4:1501-1520 PubMed41073815

2025

Microtubule forces drive nuclear damage in LMNA cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Amiad Pavlov,Julie Heffler,Carmen Suay-Corredera,Mohammad Dehghany,Kaitlyn M Shen,Noam Zuela-Sopilniak,Rani Randell,Keita Uchida,Rajan Jain,Vivek Shenoy,Jan Lammerding,Benjamin Prosser

Cell death & disease 16:549 PubMed40695795

2025

ILF3 promotes colorectal cancer cell resistance to ferroptosis by enhancing cysteine uptake and GSH synthesis via stabilizing SLC3A2 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhao Wang,Lu Zhu,Yubing Wang,Yue Han,Qingkun Wang,Wenqing Yang,Lei Zhao,Yu Zhang,Delong Pei,Wankun Huang,Aihua Jin,Zhenhua Lin,Junjie Piao

Frontiers in psychiatry 15:1396716 PubMed40276214

2025

Deficiency of autism susceptibility gene Trio in cerebellar Purkinje cells leads to delayed motor impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxin Wang,Yimeng Li,Dai Zhang,Wenzhi Sun,Jun Li

Scientific reports 15:6891 PubMed40011606

2025

High-intensity interval training alleviates STZ-induced muscle atrophy by restoration of nuclear positioning defects in C57BL/6 male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Masoud Rahmati,Rohollah Nikooie

EMBO reports 25:4728-4748 PubMed39317734

2024

SUN2 mediates calcium-triggered nuclear actin polymerization to cluster active RNA polymerase II.

Applications

Unspecified application

Species

Unspecified reactive species

Svenja Ulferts,Robert Grosse

NPJ microgravity 10:37 PubMed38521778

2024

Long non-coding RNA-NONMMMUT004552.2 regulates the unloading-induced bone loss through the miRNA-15b-5p/Syne1 in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zhang,Yu Jing,Ang Zhang,JiShan Liu,Heming Yang,Xiaotong Lou,Liyan Xu,Min Liu,Yikun Zhang,Jianwen Gu

Oncology 102:868-879 PubMed38442705

2024

Nesprin1 Deficiency Is Associated with Poor Prognosis of Renal Cell Carcinoma and Resistance to Sunitinib Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Takafumi Fukushima,Kohei Kobatake,Kento Miura,Kenshiro Takemoto,Ryoken Yamanaka,Ryo Tasaka,Yuki Kohada,Shunsuke Miyamoto,Yohei Sekino,Hiroyuki Kitano,Keisuke Goto,Kenichiro Ikeda,Akihiro Goriki,Keisuke Hieda,Osamu Kaminuma,Nobuyuki Hinata

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305867 PubMed38161226

2024

Coupling of Perinuclear Actin Cap and Nuclear Mechanics in Regulating Flow-Induced Yap Spatiotemporal Nucleocytoplasmic Transport.

Applications

Unspecified application

Species

Unspecified reactive species

Tianxiang Ma,Xiao Liu,Haoran Su,Qiusheng Shi,Yuan He,Fan Wu,Chenxing Gao,Kexin Li,Zhuqing Liang,Dongrui Zhang,Xing Zhang,Ke Hu,Shangyu Li,Li Wang,Min Wang,Shuhua Yue,Weili Hong,Xun Chen,Jing Zhang,Lisha Zheng,Xiaoyan Deng,Pu Wang,Yubo Fan

Journal of translational medicine 21:690 PubMed37840136

2023

Systematic in vivo candidate evaluation uncovers therapeutic targets for LMNA dilated cardiomyopathy and risk of Lamin A toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Chia Yee Tan,Pui Shi Chan,Hansen Tan,Sung Wei Tan,Chang Jie Mick Lee,Jiong-Wei Wang,Shu Ye,Hendrikje Werner,Ying Jie Loh,Yin Loon Lee,Matthew Ackers-Johnson,Roger S Y Foo,Jianming Jiang

Cell reports 42:112534 PubMed37210724

2023

Activation of endoplasmic reticulum stress in premature aging via the inner nuclear membrane protein SUN2.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Vidak,Leonid A Serebryannyy,Gianluca Pegoraro,Tom Misteli

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nesprin1/Syne-1 antibody [EPR14196]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (20)

Product promise