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AB74261

Anti-Nesprin3 antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal Nesprin3 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SYNE3.
1 이미지
Western blot - Anti-Nesprin3 antibody (AB74261)
  • WB

Unknown

Western blot - Anti-Nesprin3 antibody (AB74261)

All lanes:

Western blot - Anti-Nesprin3 antibody (ab74261) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 5 µg

Lane 2:

Extracts from Jurkat cells plus 5µg immunizing peptide at 5 µg

Predicted band size: 112 kDa

Observed band size: 112 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SYNE3. The exact immunogen used to generate this antibody is proprietary information.

Q6ZMZ3

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대체품

Primary Antibodies

AB186746

Anti-Nesprin3 antibody [EPR15623]

RabMAb|Recombinant

Western blot - Anti-Nesprin3 antibody [EPR15623] (AB186746)

  • 0
  • 0
Primary Antibodies

AB123031

Anti-Nesprin3 antibody [Nsp3]

Immunocytochemistry/ Immunofluorescence - Anti-Nesprin3 antibody [Nsp3] (AB123031)

  • 4
  • 1
Primary Antibodies

AB186751

Anti-Nesprin3 antibody [EPR15623-5]

RabMAb|Recombinant

Western blot - Anti-Nesprin3 antibody [EPR15623-5] (AB186751)

  • 0
  • 0
Primary Antibodies

AB190010

Anti-Nesprin3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin3 antibody (AB190010)

  • 0
  • 0

보완 제품

Primary Antibodies

AB192234

Anti-Nesprin1/Syne-1 antibody [EPR14196]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (AB192234)

  • 0
  • 0
Primary Antibodies

AB124916

Anti-SUN2 antibody [EPR6557]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (AB124916)

  • 5
  • 6
Primary Antibodies

AB156871

Anti-Emerin antibody [EPR11071]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] (AB156871)

  • 0
  • 0
Primary Antibodies

AB124770

Anti-SUN1 antibody [EPR6554]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (AB124770)

  • 5
  • 6
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific immunogen.
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Nesprin-3 also known as SYNE3 is a mechanical adaptor protein of around 215 kDa. It belongs to the spectrin repeat family and is primarily expressed in the outer nuclear membrane. This protein anchors the nucleus to the cytoskeleton by interacting with cytoskeletal linkers like plectin. It plays a role in maintaining the structural integrity of the nucleus and cellular architecture due to these anchoring functions.
Biological function summary

Nesprin-3 is involved in the linking of the nuclear envelope to the cytoskeleton and it forms part of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. This complex is important for nuclear movement within cells and has a role in signal transduction from the cell surface to the nucleus. Through its ability to bind to other cytoskeletal elements Nesprin-3 contributes to force transmission across the cell impacting cell polarity and migration.

Pathways

Nesprin-3 interacts with several cytoskeletal signaling pathways particularly the actin and intermediate filament pathways. It associates with proteins like plectin and vimentin playing significant parts in cellular mechanics and signaling. By engaging in these pathways Nesprin-3 helps control cellular responses to mechanical stress and influences various processes including cell migration and division.

Nesprin-3 has been linked to muscular dystrophies and certain cancers. In muscular dystrophies its interactions with plectin can become disrupted leading to compromised muscle integrity and function. In cancer changes in its expression or interactions may affect cell movement and invasion contributing to metastasis. Its role in these diseases highlights its importance in maintaining proper cell architecture and function.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.
See full target information SYNE3

대체 명칭 보기

C14orf139, C14orf49, LINC00341, SYNE3, Nesprin-3, KASH domain-containing protein 3, Nuclear envelope spectrin repeat protein 3, KASH3
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 7:eabg6908 PubMed34524844

2021

MYO10 drives genomic instability and inflammation in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Franklin Mayca Pozo,Xinran Geng,Ilaria Tamagno,Mark W Jackson,Ernest G Heimsath,John A Hammer,Richard E Cheney,Youwei Zhang

Science advances 7: PubMed33523987

2021

The nuclear piston activates mechanosensitive ion channels to generate cell migration paths in confining microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Pyo Lee,Farid Alisafaei,Kolade Adebawale,Julie Chang,Vivek B Shenoy,Ovijit Chaudhuri

Cell metabolism 30:903-916.e7 PubMed31523006

2019

Nuclear Glycogenolysis Modulates Histone Acetylation in Human Non-Small Cell Lung Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ramon C Sun,Vikas V Dukhande,Zhengqiu Zhou,Lyndsay E A Young,Shane Emanuelle,Christine Fillmore Brainson,Matthew S Gentry

Oncology reports 40:783-792 PubMed29901114

2018

Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ji,Jinquan Jiang,Lihua Huang,Wei Feng,Zhang Zhang,Longyu Jin,Xiaowei Xing

Proceedings of the National Academy of Sciences of 107:5076-81 PubMed20145110

2010

Abnormal development of the cerebral cortex and cerebellum in the setting of lamin B2 deficiency.

Applications

ICC/IF, WB

Species

Mouse, Mouse

Catherine Coffinier,Sandy Y Chang,Chika Nobumori,Yiping Tu,Emily A Farber,Julia I Toth,Loren G Fong,Stephen G Young
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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