AB221660

Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker

Name: Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker
Brand: Abcam Limited
SKU: AB221660
Rating: 1 (1 reviews)

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(35 제품이 사용된 논문 )

Anti-Nestin antibody [EPR22023] (ab221660) is a rabbit monoclonal antibody detecting Nestin in Western Blot, IHC-P, IHC-Fr. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

6 이미지

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Nestin with ab221660 at 1/100 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in glomeruli of mouse kidney (PMID : 17210924) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Nestin with ab221660 at 1/100 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in glomeruli of rat kidney (PMID : 17210924) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of glomerulus and stromal cells in mouse kidney (PMID : 17210924) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in mouse lung (PMID : 17210924) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemical analysis of paraffin-embedded mouse embryonic day 14.5 cerebrum tissue labeling Nestin with ab221660 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on cerebrum of embryonic day 14.5 mouse (PMID : 16728475) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure times : Lane 1 : 26 seconds; Lane 2 : 81 seconds; Lane 3 : 15 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID : 23319587, PMID : 23407796).

All lanes:

Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (ab221660) at 1/1000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse P0 brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 177 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22023

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Perform heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Perform heat-mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-Nestin antibody [EPR22023] (ab221660) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.

What is the molecular weight of Nestin?
Anti-Nestin [EPR22023] (ab221660) specifically detects a band for Nestin (UniProt: Q6P5H2) at a molecular weight of 177kDa.

Trusted by the scientific community
Anti-Nestin [EPR22023] (ab221660) was first used in a scientific publication in 2018 and has been cited over 20 times in peer-reviewed journals.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.

Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

See full target information Nes

대체 명칭 보기

Nestin, Nes

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제품이 사용된 논문 (35)

Recent publications for all applications. Explore the 전체 목록and refine your search

Biomaterials research 29:0256 PubMed40980288

2025

Decreasing Lamin A Triggers Cell Fate Transitions through Heterochromatin-Nuclear Periphery Detethering.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Sun,Yafan Xie,Zhaoyan Zuo,Jian Liu,Jiaqi Yang,Iqra Ali,Qin Peng,Juhui Qiu

Acta neuropathologica communications 13:158 PubMed40684195

2025

Unraveling the development of cutaneous neurofibromas in neurofibromatosis type 1.

Applications

Unspecified application

Species

Unspecified reactive species

Pernelle Pulh,Fanny Coulpier,Audrey Onfroy,Layna Oubrou,Wanzhen Zhang,Léa Toledano,Elie Abou Zougheib,Laura Fertitta,Pierre Wolkenstein,Piotr Topilko

Cell reports. Medicine 6:102052 PubMed40239632

2025

Catalytic neural stem cell exosomes for multi-stage targeting and synergistical therapy of retinal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqiang Yang,Xiaojun Wang,Diwei Zheng,Jing Feng,Wenjun Kong,Yue Li,Guanghui Ma,Wei Wei,Yong Tao

Translational stroke research 16:1577-1593 PubMed39853651

2025

Neuroprotective Effects, Mechanisms of Action and Therapeutic Potential of the Kv7/KCNQ Channel Opener QO-83 in Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Huiran Zhang,Yanfei Tian,Yan Zhang,Yan Wang,Jinlong Qi,Xiangyu Wang,Yi Yuan,Rong Chen,Yupeng Zhao,Chang Liu,Najing Zhou,Lanxin Liu,Han Hao,Xiaona Du,Hailin Zhang

Journal of advanced research 74:429-442 PubMed39353531

2024

Gastrodin attenuates high fructose-induced sweet taste preference decrease by inhibiting hippocampal neural stem cell ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan-Feng Tang,Hong Ding,Ya-Qian Wu,Zi-An Miao,Zi-Xuan Wang,Wen-Xuan Wang,Ying Pan,Ling-Dong Kong

Bone research 12:34 PubMed38816384

2024

Lumbar instability remodels cartilage endplate to induce intervertebral disc degeneration by recruiting osteoclasts via Hippo-CCL3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hanwen Li,Yingchuang Tang,Zixiang Liu,Kangwu Chen,Kai Zhang,Sihan Hu,Chun Pan,Huilin Yang,Bin Li,Hao Chen

Neural regeneration research 20:557-573 PubMed38819067

2024

Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiewen Chen,Xiaolin Zeng,Le Wang,Wenwu Zhang,Gang Li,Xing Cheng,Peiqiang Su,Yong Wan,Xiang Li

BioMed research international 2024:2973407 PubMed38449509

2024

Identifying G6PC3 as a Potential Key Molecule in Hypoxic Glucose Metabolism of Glioblastoma Derived from the Depiction of F-Fluoromisonidazole and F-Fluorodeoxyglucose Positron Emission Tomography.

Applications

Unspecified application

Species

Unspecified reactive species

Michinari Okamoto,Shigeru Yamaguchi,Ryosuke Sawaya,Sumire Echizenya,Yukitomo Ishi,Sadahiro Kaneko,Hiroaki Motegi,Takuya Toyonaga,Kenji Hirata,Miki Fujimura

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38383496

2024

SMARCA4 Loss and Mutated β-Catenin Induce Proliferative Lesions in the Murine Embryonic Cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Göbel,Melanie Schoof,Dörthe Holdhof,Michael Spohn,Ulrich Schüller

Journal of nanobiotechnology 22:66 PubMed38368393

2024

Exosome lncRNA IFNG-AS1 derived from mesenchymal stem cells of human adipose ameliorates neurogenesis and ASD-like behavior in BTBR mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Fu,Yuan-Lin Zhang,Rong-Qi Liu,Meng-Meng Xu,Jun-Ling Xie,Xing-Liao Zhang,Guang-Ming Xie,Yao-Ting Han,Xin-Min Zhang,Wan-Ting Zhang,Jing Zhang,Jun Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

Western blot - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

관련 conjugated 항체와 다양한 조성의 항체 (6)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (35)

Product promise