AB113202

Anti-NET1 antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal NET1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NET1 aa 500-600.

대체 명칭 보기

ARHGEF8, NET1, Neuroepithelial cell-transforming gene 1 protein, Proto-oncogene p65 Net1, Rho guanine nucleotide exchange factor 8

3 이미지

Unknown

Immunoprecipitation - Anti-NET1 antibody (AB113202)

Immunoprecipitation of NET1 (6 μg of antibody per mg of Jurkat cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-Net1 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with ab113202; Lane 3- Immunoprecipitation with IgG control. Anti-Net 1 antibody (ab113202) used at 1 μg/ml for western blot. Detection : Chemiluminescence with exposure time of 3 min.

All lanes:

Immunoprecipitation - Anti-NET1 antibody (ab113202)

Predicted band size: 68 kDa

false

Unknown

Immunoprecipitation - Anti-NET1 antibody (AB113202)

Immunoprecipitation of NET1 (6 μg of antibody per mg of HEK-293T cell lysate; 20% of the IP loaded). Anti-Net 1 antibody (ab113202) used at 1 μg/ml for western blot. Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-NET1 antibody (ab113202)

Predicted band size: 68 kDa

false

Unknown

Western blot - Anti-NET1 antibody (AB113202)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-NET1 antibody (ab113202) at 0.1 µg/mL

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 68 kDa

false

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NET1 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

Q7Z628

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NET1 or Neuroepithelial Transforming Gene 1 is a fascinating protein often identified by scientists because of its role in cellular dynamics. NET1 weighs approximately 64 kDa and functions mainly as a guanine nucleotide exchange factor (GEF) for RhoA a small GTPase. NET1 facilitates the conversion of inactive GDP-bound RhoA into its active GTP-bound form. NET1 prominently expresses in several tissues including the liver and kidneys. Researchers often focus on NET1's mechanical action in connection to its influence on the cytoskeleton and cellular movement.

Biological function summary

The primary function of NET1 revolves around its ability to regulate RhoA activity significantly impacting cell shape and mobility. NET1 plays a role in actin cytoskeleton reorganization making it important to processes such as cell migration and adhesion. NET1 acts within a protein complex that includes RhoA and other neighboring molecules like GTPase-activating proteins and guanine nucleotide dissociation inhibitors. This interaction highlights its importance for cellular structures and functions connected to movement and stability.

Pathways

NET1 engages with cellular pathways controlling cytoskeleton organization and cellular migration. It prominently participates in the Rho signaling pathway a critical regulator of the actin cytoskeleton. NET1's activity in these pathways associates closely with proteins such as ROCK a Rho-associated kinase that influences cytoskeletal rearrangements. The connection of NET1 with focal adhesion pathways showcases its effective role in anchoring cells to their surroundings and responding to mechanical cues.

NET1 exhibits significant links with cancer and fibrosis. NET1 overexpression correlates with increased invasiveness and metastatic potential in various cancer types including breast and gastric cancer. Researchers note NET1's connection to epithelial-mesenchymal transition (EMT) a process vital for cancer progression. Furthermore NET1 involvement extends to fibrotic disorders where excessive connective tissue forms. In these diseases NET1 interacts with fibrotic proteins like collagen indicating its contribution to pathological tissue remodeling.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

See full target information NET1

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 44:115237 PubMed39883578

2025

G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Liana C Boraas,Mengwei Hu,Pieter Martino,Lauren Thornton,Charles E Vejnar,Gang Zhen,Longhui Zeng,Dylan M Parker,Andy L Cox,Antonio J Giraldez,Xiaolei Su,Christine Mayr,Siyuan Wang,Stefania Nicoli

Molecular medicine (Cambridge, Mass.) 30:272 PubMed39719558

2024

Doublecortin regulates the mitochondrial-dependent apoptosis in glioma via Rho-A/Net-1/p38-MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Iqra Nadeem,Zhou Han,Hong Xiaoliang,Seyram Yao Adzraku,Piniel Alphayo Kambey,Kouminin Kanwore,Mu Peipei,Adebayo Oluwafemi Adekunle,Joseph Adu-Amankwaah,Abiola Abdulrahman Ayanlaja,Yi Zheng,Gao Dianshuai,Xiaomei Liu,Yuanjian Song

Bioengineered 12:1699-1707 PubMed33955315

2021

Baicalin suppresses lung cancer growth phenotypes via miR-340-5p/NET1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fucheng Zhao,Zhenxia Zhao,Yanru Han,Sujuan Li,Caili Liu,Kui Jia

Oncology letters 16:2893-2902 PubMed30127877

2018

VTIQ evaluates antitumor effects of NET-1 siRNA by UTMD in HCC xenograft models.

Applications

Unspecified application

Species

Unspecified reactive species

Xitian Liang,Bolin Wu,Haitao Shang,Xue Han,Hui Jing,Yixin Sun,Wen Cheng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NET1 antibody

Immunoprecipitation - Anti-NET1 antibody (AB113202)

Immunoprecipitation - Anti-NET1 antibody (AB113202)

Western blot - Anti-NET1 antibody (AB113202)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise