Anti-NeuroD1 antibody [HL3356] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [HL3356] - BSA and Azide free (AB327519)

ab327519 detects NeuroD1 protein by immunohistochemical analysis.
Sample : Paraffin-embedded mouse hippocampus.
NeuroD1 stained by ab327519 diluted at 1 : 200.
Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [HL3356] - BSA and Azide free (AB327519)

ab327519 detects NeuroD1 protein by immunofluorescent analysis.
Sample : DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : NeuroD1 stained by ab327519 diluted at 1 : 250.
Red : Tau, an axon marker, stained by Tau antibody diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

• WB

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Western blot - Anti-NeuroD1 antibody [HL3356] - BSA and Azide free (AB327519)

IMR32 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327519 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-NeuroD1 antibody [HL3356] - BSA and Azide free (ab327519) at 1/1000 dilution

Lane 1:

IMR32 whole cell extracts at 30 µg

Lane 2:

IMR32 nuclear extract at 30 µg

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• WB

Supplier Data

Western blot - Anti-NeuroD1 antibody [HL3356] - BSA and Azide free (AB327519)

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327519 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-NeuroD1 antibody [HL3356] - BSA and Azide free (ab327519) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue extracts at 50 µg

Lane 2:

Mouse muscle tissue extracts at 50 µg

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