Anti-Neurofilament heavy polypeptide antibody [3G3]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (AB19386)

Immunohistochemistry analysis of Neurofilament, Heavy chain showing staining in the cytoplasm and axons of formalin-fixed, paraffin-embedded human brain tissue (B) and magnified section (C) compared with an isotype control (A). To expose target proteins, antigen retrieval was performed using HEIR with a buffer (pH 6.2). Tissues were probed with a Neurofilament, Heavy chain monoclonal antibody for 60 minutes at a dilution of 2 μg/mL and detection was performed using an HRP-conjugated detection system for 30 minutes followed by DAB staining.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [3G3] (AB19386)

Immunofluorescence of neurofilament, heavy chain in rat cerebral cortex cultures in green.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (AB19386)

Immunohistochemistry analysis of paraffin-embedded rat brain tissue (right) compared to a negative control without primary antibody (left) labelling Neurofilament Heavy Chain with ab19386. Positive staining in the cytoplasm and nucleus (right).

To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament Heavy Chain monoclonal antibody diluted in 3% BSA-PBS at 1/20 dilution overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• WB

Supplier Data

Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (AB19386)

Results show a band at ~200 kDa.

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386) at 1/500 dilution

Lane 1:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 25 µg

Lane 2:

Rat brain tissue lysates at 25 µg

Secondary

All lanes:

HRP-conjugated secondary antibody

Predicted band size: 112 kDa

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