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AB277260

Anti-Neurogranin antibody [EPR22523-107] - BSA and Azide free (Detector)

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Anti-Neurogranin antibody [EPR22523-107] - BSA and Azide free (Detector) (ab277260) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Neurogranin in ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
2 이미지
Sandwich ELISA - Anti-Neurogranin antibody [EPR22523-107] - BSA and Azide free (Detector) (AB277260)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Neurogranin antibody [EPR22523-107] - BSA and Azide free (Detector) (AB277260)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab277083).

Sandwich ELISA - Anti-Neurogranin antibody [EPR22523-107] - BSA and Azide free (Detector) (AB277260)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Neurogranin antibody [EPR22523-107] - BSA and Azide free (Detector) (AB277260)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab277084).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22523-107

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB277083

Human Neurogranin ELISA Kit (NRGN)

Recombinant|SimpleStep

ELISA - Human Neurogranin ELISA Kit (NRGN) (AB277083)

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Sample Prep & Detection Kits

AB201795

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®

ELISA - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (AB201795)

  • 0
  • 0
Sample Prep & Detection Kits

AB188290

Gold-Maleimide Conjugation Kit (40nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (40nm, 20 OD) (AB188290)

  • 0
  • 0
Proteins & Peptides

AB316609

Recombinant Human Neurogranin Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Neurogranin Protein Standard (His tag) (AB316609)

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Primary Antibodies

AB245249

Anti-LINE-1 ORF1p antibody [EPR22227-6]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (AB245249)

  • 0
  • 0
Primary Antibodies

AB245031

Anti-S100 beta antibody [EPR20510-83] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-S100 beta antibody [EPR20510-83] - BSA and Azide free (Detector) (AB245031)

  • 0
  • 0
Primary Antibodies

AB281197

Anti-GAP43 antibody [EPR23863-12] - BSA and Azide free (Capture)

RabMAb|Recombinant

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab277260 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Neurogranin. The recommended pair for sandwich ELISA is:

Capture:

ab277259

, Human Neurogranin Capture Antibody (unconjugated)

Detector: ab277260, Human Neurogranin Detector Antibody (unconjugated)

The reference range value is 9.375 - 600 pg/ml.

Alternatively, you can use:
Capture: ab277261, Human Neurogranin (Trunc P75) Capture Antibody (unconjugated)
Detector: ab277260, Human Neurogranin Detector Antibody (unconjugated)
The reference range value is 25 - 400 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Neurogranin also known as NRGN is a small neuronal protein with an approximate molecular mass of 7.6 kDa. It is mainly expressed in the brain predominantly within the dendrites of neurons. Neurogranin binds to calmodulin in the presence of calcium playing an important role in synaptic plasticity. This protein acts as a kinase substrate influencing signal transduction pathways tied to learning and memory. Due to its specific expression pattern neurogranin serves as a significant molecular marker for studying the nervous system.
Biological function summary

Neurogranin functions as a modulator in cerebellar signaling and synaptic transmission. It influences calcium-calmodulin signaling facilitating the activation of proteins important in the central nervous system. Neurogranin does not form part of a multi-protein complex but interacts closely with calmodulin to regulate downstream signal cascades. These interactions emphasize its fundamental role in modulating processes that influence cognitive functions like learning and memory.

Pathways

Neurogranin significantly impacts the calcium signaling pathway and contributes to the regulation of long-term potentiation. It closely associates with proteins such as Protein Kinase C (PKC) and calmodulin-dependent protein kinases which are instrumental in these pathways. By modulating these essential signaling routes neurogranin affects intracellular signals that are vital for synaptic plasticity and enhanced neural communication.

Neurogranin has a noted involvement in conditions like Alzheimer's disease and cognitive impairment. Changes in neurogranin expression levels are linked to the progression of Alzheimer's where reduced levels may contribute to the disruption of synaptic plasticity. Interactions with amyloid-beta peptides and other proteins such as tau highlight its potential as a biomarker for neurodegenerative diseases. These connections underline neurogranin's significant role in understanding cognitive-related disorders and guiding therapeutic research.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).
See full target information NRGN

대체 명칭 보기

Neurogranin, Ng, RC3, NRGN
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Product promise

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