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AB47378

Anti-NF2 / Merlin (phospho S518) antibody

2

(3 리뷰들)

|

(1 출판물)

Rabbit Polyclonal NF2 / Merlin phospho S518 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NF2 pS518 aa 450-550.
1 이미지
Western blot - Anti-NF2 / Merlin (phospho S518) antibody (AB47378)
  • WB

Unknown

Western blot - Anti-NF2 / Merlin (phospho S518) antibody (AB47378)

All lanes:

Western blot - Anti-NF2 / Merlin (phospho S518) antibody (ab47378) at 1/500 dilution

Lane 1:

HuvEc cell extracts treated with IFN-a(1000U/ml, 18 hours)

Lane 2:

HuvEc cell extracts

Predicted band size: 70 kDa

Observed band size: 69 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NF2 pS518 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

P35240

Specificity

Detects endogenous levels of Merlin only when phosphorylated at serine 518.

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대체품

Primary Antibodies

AB131473

Anti-NF2 / Merlin (phospho S518) antibody

Immunocytochemistry/ Immunofluorescence - Anti-NF2 / Merlin (phospho S518) antibody (AB131473)

  • 0
  • 0
Primary Antibodies

AB2478

Anti-NF2 / Merlin (phospho S518) antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin (phospho S518) antibody (AB2478)

  • 4
  • 1
Primary Antibodies

AB217016

Anti-NF2 / Merlin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin antibody (AB217016)

  • 0
  • 0
Primary Antibodies

AB84550

Anti-NF2 / Merlin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin antibody (AB84550)

  • 0
  • 0
Primary Antibodies

AB109244

Anti-NF2 / Merlin antibody [EPR2573(2)]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (AB109244)

  • 0
  • 0

보완 제품

Primary Antibodies

AB308025

Anti-NF2 / Merlin antibody [RP23040199]

Recombinant

  • 0
  • 0
Primary Antibodies

AB70561

Anti-LATS1/WARTS antibody

Immunoprecipitation - Anti-LATS1/WARTS antibody (AB70561)

  • 4
  • 1
Primary Antibodies

AB205270

Anti-active YAP1 antibody [EPR19812]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-active YAP1 antibody [EPR19812] (AB205270)

  • 5
  • 25
Primary Antibodies

AB76252

Anti-YAP1 (phospho S127) antibody [EP1675Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)

  • 4
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The NF2 gene produces a protein known as Neurofibromin 2 often called Merlin. The NF2 protein or Merlin protein plays a mechanical role in the cell as a tumor suppressor by stabilizing cellular structure and regulating cell growth. Its molar mass is approximately 70 kDa. NF2 protein is found mainly in the nervous system particularly in Schwann cells which are important for nerve function.
Biological function summary

The NF2 protein functions to control cell proliferation differentiation and motility. It associates with the cytoskeleton acting as a bridge between cell surface proteins and the actin cytoskeleton. Merlin as part of the ERM (ezrin radixin moesin) family contributes to the assembly of protein complexes at the cell membrane. Through its interactions it plays a significant role in maintaining proper cell signaling and structural integrity.

Pathways

NF2 protein integrates into the Hippo signaling pathway which regulates organ size by controlling cell growth and apoptosis. Within this pathway Merlin interacts with members such as YAP1 and LATS1/2 moderating their activity to prevent over-proliferation of cells. NF2 also connects with the mTOR pathway which is critical for cell metabolism and growth further emphasizing its regulatory functions in cellular homeostasis.

Mutations or deletions in the NF2 gene are associated with neurofibromatosis type 2 a condition that causes benign tumors on nerve tissues known as schwannomas. NF2 protein is linked to other proteins such as angiogenesis factors contributing to the development of these tumors. Schwannomatosis another disorder related to NF2 involves nerve sheath tumor formation with Merlin mutation playing a role indicating its importance in nervous system health.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
See full target information NF2 pS518

대체 명칭 보기

SCH, NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer research 67:520-7 PubMed17234759

2007

Merlin/neurofibromatosis type 2 suppresses growth by inhibiting the activation of Ras and Rac.

Applications

WB

Species

Human

Helen Morrison,Tobias Sperka,Jan Manent,Marco Giovannini,Helmut Ponta,Peter Herrlich
제품이 사용된 논문 모두 보기
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Product promise

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