- IP
Supplier Data
Immunoprecipitation - Anti-NFIB / NF1B2 antibody (AB264332)
NFIB / NF1B2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264332 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264332 at 1 μg/ml.
Lane 1 : ab264332 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in Hela whole cell lysate.
Exposure time : 3 seconds.
All lanes:
Immunoprecipitation - Anti-NFIB / NF1B2 antibody (ab264332)
Predicted band size: 47 kDa
false
- WB
Supplier Data
Western blot - Anti-NFIB / NF1B2 antibody (AB264332)
All lanes:
Western blot - Anti-NFIB / NF1B2 antibody (ab264332) at 0.1 µg/mL
Lane 1:
HeLa human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2:
HeLa whole cell lysate at 15 µg
Lane 3:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 47 kDa
true
Exposure time: 30s
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