AB229829
Rabbit Polyclonal NFS1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFS1 aa 200 to C-terminus.
대체 명칭 보기
NIFS, HUSSY-08, NFS1, Cysteine desulfurase
1 이미지
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFS1 antibody (AB229829)
Paraffin-embedded human colon cancer tissue stained for NFS1 using ab229829 at 1/100 dilution in immunohistochemical analysis.
대체품
보완 제품
![Western blot - Anti-FECH antibody [EPR8312] (AB137042)](https://content.abcam.com/products/images/fech-antibody-epr8312-ab137042--western-blot-img57435.jpg)
Primary Antibodies
AB154860
Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker
RabMAb|Recombinant|Lab Essentials|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (AB154860)](https://content.abcam.com/products/images/alas1-antibody-epr10247-mitochondrial-marker-ab154860--immunocytochemistry-immunofluorescence-img23113.jpg)
- 5
- 2
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
특성 및 보관 정보
제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
제품 프로토콜
For this product, it's our understanding that no specific protocols are required. You can visit:
- Visit the General protocols
- Visit the Troubleshooting
타겟 정보
Isoform Mitochondrial. Cysteine desulfurase, of the core iron-sulfur cluster (ISC) assembly complex, that catalyzes the desulfuration of L-cysteine to L-alanine, as component of the cysteine desulfurase complex, leading to the formation of a cysteine persulfide intermediate at the active site cysteine residue and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (PubMed : 18650437, PubMed : 29097656, PubMed : 31101807). The persulfide is then transferred on the flexible Cys loop from the catalytic site of NFS1 to the surface of NFS1 (PubMed : 29097656). After the NFS1-linked persulfide sulfur is transferred to one of the conserved Cys residues of the scaffold, a reaction assisted by FXN (By similarity). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1 : LYRM4 : NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity).. Isoform Cytosolic. May catalyze the desulfuration of L-cysteine to L-alanine as component of the cysteine desulfurase complex (NFS1 : LYRM4), leading to the formation of a cysteine persulfide intermediate (PubMed : 16527810, PubMed : 18650437). Acts as a sulfur donor for MOCS3 by transferring the sulfur of the cysteine persulfide intermediate on MOCS3 (PubMed : 18650437, PubMed : 23593335).
제품이 사용된 논문 (3)
Recent publications for all applications. Explore the 전체 목록 and refine your search
The Journal of biological chemistry 301:110234 PubMed40378953
2025
Antioxidant capacity of the iron-sulfur cluster assembly protein IscU2 is mediated by aspartate metabolism to promote tumor survival.
Applications
Unspecified application
Species
Unspecified reactive species
Xunjun Yang,Na Liang,Dandan Liu,Jimei Yan,Xiali Yang,Jinya Lv,Saijun Xiao,Xiujuan Wei,Xuyang Chen,Zhengquan Yang,Shanying Gui,Liqin Jin,Shihui Yu,Jianxin Lyu,Xiaojun Ren
eLife 10: PubMed34612205
2021
Divergent acyl carrier protein decouples mitochondrial Fe-S cluster biogenesis from fatty acid synthesis in malaria parasites.
Applications
Unspecified application
Species
Unspecified reactive species
Seyi Falekun,Jaime Sepulveda,Yasaman Jami-Alahmadi,Hahnbeom Park,James A Wohlschlegel,Paul A Sigala
Neoplasma 66:564-575 PubMed30943745
2019
LncRNA TTN-AS1 contributes to gastric cancer progression by acting as a competing endogenous RNA of miR-376b-3p.
Applications
Unspecified application
Species
Unspecified reactive species
M M Dong,S J Peng,Y N Yuan,H P Luo
Product promise
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