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AB236072

Anti-NgBR antibody [EPR8668] - BSA and Azide free

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Rabbit Recombinant Monoclonal NGBR antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

C6orf68, NGBR, NUS1, Dehydrodolichyl diphosphate synthase complex subunit NUS1, Cis-prenyltransferase subunit NgBR, Nogo-B receptor, Nuclear undecaprenyl pyrophosphate synthase 1 homolog, NgBR

1 이미지
Western blot - Anti-NgBR antibody [EPR8668] - BSA and Azide free (AB236072)
  • WB

Unknown

Western blot - Anti-NgBR antibody [EPR8668] - BSA and Azide free (AB236072)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168351).

All lanes:

Western blot - Anti-NgBR antibody [EPR8668] (<a href='/ko/products/primary-antibodies/ngbr-antibody-epr8668-ab168351'>ab168351</a>) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8668

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB252149

Alexa Fluor® 488 Anti-CD4 antibody [EPR20122]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (AB252149)

  • 0
  • 0
Primary Antibodies

AB303467

Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)

  • 0
  • 0
Primary Antibodies

AB47085

Anti-Nogo A+B antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nogo A+B antibody (AB47085)

  • 5
  • 10
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

ab236072 is the carrier-free version of ab168351.

This antibody was developed as part of a collaboration between Epitomics, the National Institutes of Health and the lab of Qing (Robert) Miao from the Medical College of Wisconsin.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NgBR also known as the Nogo-B receptor is a transmembrane protein with an approximate mass of 38 kDa. It plays a role in cellular mechanisms especially within endothelial cells where it is expressed abundantly. NgBR involves itself in modulating the activity and localization of certain enzymes particularly in processes requiring lipid modification. Expression of NgBR can also be found in other tissues suggesting a broader range of functions.
Biological function summary

NgBR engages in various cellular operations influencing development survival and migration of cells. It acts as a cofactor for enzymes participating in dolichol biosynthesis and cholesterol synthesis. NgBR often forms a complex with other proteins to execute these roles. By interacting with Nogo-B NgBR impacts key angiogenesis and vascular homeostasis decisions playing part in growth and maintenance of blood vessels.

Pathways

NgBR aligns itself with the PI3K-Akt signaling cascade and the Ras pathway both important in cellular proliferation and survival. In these pathways NgBR associates closely with PI3K and interacts with Ras proteins enabling effective signal transduction. NgBR's contribution helps maintain cellular responses to growth signals and ensures proper functioning of cellular mechanisms.

Altered expression or function of NgBR associates with various pathologies including cancer and cardiovascular diseases. NgBR's connection to cancer lies in its involvement with endothelial survival and proliferation with disruptions potentially leading to tumor growth and metastasis. In cardiovascular disease NgBR plays a role in endothelial dysfunction linking with proteins such as Nogo-B which influences blood vessel repair and formation hinting at implications in atherosclerosis and related disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery (PubMed : 21572394, PubMed : 25066056, PubMed : 28842490, PubMed : 32817466, PubMed : 33077723). Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER) (PubMed : 21572394, PubMed : 25066056, PubMed : 28842490, PubMed : 32817466, PubMed : 33077723). Synthesizes long-chain polyprenols, mostly of C95 and C100 chain length (PubMed : 32817466). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol (PubMed : 21572394). Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator (PubMed : 16835300).
See full target information NUS1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 12:785941 PubMed35111156

2022

Exosomes Recovered From the Plasma of COVID-19 Patients Expose SARS-CoV-2 Spike-Derived Fragments and Contribute to the Adaptive Immune Response.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Pesce,Nicola Manfrini,Chiara Cordiglieri,Spartaco Santi,Alessandra Bandera,Andrea Gobbini,Paola Gruarin,Andrea Favalli,Mauro Bombaci,Alessandro Cuomo,Federica Collino,Giulia Cricrì,Riccardo Ungaro,Andrea Lombardi,Davide Mangioni,Antonio Muscatello,Stefano Aliberti,Francesco Blasi,Andrea Gori,Sergio Abrignani,Raffaele De Francesco,Stefano Biffo,Renata Grifantini
제품이 사용된 논문 모두 보기
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Product promise

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