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AB288434

Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192]

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Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (ab288434) is a rat monoclonal antibody detecting Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 in Western Blot, ICC/IF. Suitable for Human, Mouse.

- Recombinant format for unrivalled batch-batch consistency
1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (AB288434)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (AB288434)

The image data was generated using the chimeric mouse version of the same antibody clone

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, stained for muscle acetylcholine receptor using ab288434 at 10μg/ml for 1 hr. Followed by Alexa Fluor® 488 secondary antibody (2 μg/ml), showing positive cytoplasmic staining.

The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab288434, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (ab288434) followed by Alexa Fluor® 488 secondary antibody.

주요 정보

Host species

Rat

Clonality

Monoclonal

Clone number

mAb 192

Isotype

IgG2b

Light chain 타입

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CHRNA1.

P02708

Specificity

The antibody binds to human muscle AChR with a Kd of 10 pM, to mouse muscle AChR with a Kd of 50 pM, and to rat muscle AChR with a Kd of 646 nM

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보완 제품

Primary Antibodies

AB216485

Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (AB216485)

  • 4
  • 5
Primary Antibodies

AB151627

Anti-Nicotinic Acetylcholine Receptor gamma antibody

Western blot - Anti-Nicotinic Acetylcholine Receptor gamma antibody (AB151627)

  • 0
  • 0
Primary Antibodies

AB76159

Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y]

RabMAb|Recombinant

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] (AB76159)

  • 0
  • 0
Primary Antibodies

AB259318

Anti-Aquaporin 4 antibody [EPR24281-65]

BOND RX™ Validated|KO Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR24281-65] (AB259318)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

What is this antibody validated in?
Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (ab288434) is a rat recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of Nicotinic Acetylcholine Receptor alpha 1/CHRNA1?
Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 [mAb 192] (ab288434) specifically detects a band for Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 (UniProt: P02708) at a molecular weight of 54kDa.

Other related products
We have a range of other formats of antibody clone [mAb 192] also available for your convenience: ab288434, Carrier free - ab307999
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Preservative: 0.02% Proclin 300 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor alpha 1 also known as CHRNA1 is an essential component of the nicotinic acetylcholine receptor complex. This receptor an ion channel found in the neuromuscular junction is involved in the mechanical process of signal transmission between nerves and muscles. The protein facilitates the binding of acetylcholine a neurotransmitter which triggers an ion flow causing muscle contraction. The CHRNA1 subunit generally displays a mass of approximately 58 kDa and is widely expressed in muscle tissues.
Biological function summary

The Nicotinic Acetylcholine Receptor alpha 1 has a fundamental role in neuromuscular communication. It is part of a pentameric complex in combination with other acetylcholine receptor subunits. When acetylcholine binds to the receptor the channel opens permitting cations like sodium and calcium to enter the muscle cell leading to depolarization and subsequent muscle contraction. This biological process is essential for voluntary muscle movement.

Pathways

The Nicotinic Acetylcholine Receptor alpha 1 is involved in the neuromuscular signaling pathway. This pathway regulates muscle contraction by converting neural chemical signals into mechanical responses. In this pathway CHRNA1 interacts closely with proteins such as the acetylcholine ligand and acetylcholine receptor. These interactions ensure effective synaptic transmission at the neuromuscular junction contributing to muscle activation and relaxation cycles.

Nicotinic Acetylcholine Receptor alpha 1 is linked to conditions such as myasthenia gravis and congenital myasthenic syndromes. In myasthenia gravis autoantibodies targeting CHRNA1 hinder normal receptor function on muscles leading to weakness and fatigue. Additionally the protein is associated with congenital myasthenic syndromes where mutations can alter the receptor's function resulting in impaired neuromuscular transmission. The receptor's relationship with other proteins like the acetylcholine receptor and AchR protein plays an important role in these conditions often defining the severity and specific characteristics of the disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Isoform 1. Upon acetylcholine binding, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.. Isoform 2. Non functional acetylcholine receptor alpha subunit which is not integrated into functional acetylcholine-gated cation-selective channels.
See full target information CHRNA1

대체 명칭 보기

ACHRA, CHNRA, CHRNA1, Acetylcholine receptor subunit alpha
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

FEBS letters 389:195-8 PubMed8766828

1996

Characterisation, crystallisation and preliminary X-ray diffraction analysis of a Fab fragment of a rat monoclonal antibody with very high affinity for the human muscle acetylcholine receptor.

Applications

Unspecified application

Species

Unspecified reactive species

M Kontou,E H Vatzaki,A Kokla,K R Acharya,N G Oikonomakos,S J Tzartos
제품이 사용된 논문 모두 보기
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Product promise

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