AB233717

Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10]

Name: Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10]
Brand: Abcam Limited
SKU: AB233717
Rating: 3 (1 reviews)

(1 리뷰)

(0 출판물)

Mouse Monoclonal Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHRNA3 aa 1-250.

대체 명칭 보기

NACHRA3, CHRNA3, Neuronal acetylcholine receptor subunit alpha-3

6 이미지

Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cells labeling Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution (green) compared with a negative control (red).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Nicotinic Acetylcholine Receptor alpha using ab233717 at 1/200 dilution (green) compared to a negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
Blue : DRAQ5 fluorescent DNA dye.
Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

SMMC-7721 (human hepatocarcinoma cell line) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
Blue : DRAQ5 fluorescent DNA dye.
Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.

Lane 2:

Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate.

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

Expected MW is 50.6 kDa.

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution

All lanes:

Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein

Predicted band size: 57 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

6D3A10

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human CHRNA3 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P32297

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from tissue culture supernatant.

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor alpha 3 also known as CHRNA3 is a subunit of the nicotinic acetylcholine receptors important for mediating synaptic transmission. This protein with a mass of approximately 57 kDa forms one part of a pentameric complex which functions as an ion channel. It is widely expressed in the central and peripheral nervous systems contributing to the signaling mechanisms in neurons. CHRNA3 in conjunction with other nicotinic receptor subunits plays a role in neurotransmitter release exerting control over calcium and sodium ion influx that affects neuron excitability and response.

Biological function summary

CHRNA3 serves as a critical element of neurotransmission playing a part in the nicotine-induced dopaminergic signaling pathways. Often forming a complex with other subunits like beta-2 and beta-4 it modulates synaptic plasticity and influences behavioral processes such as addiction and attention. This subunit composition affects receptor specificity and sensitivity determining how neurons respond to acetylcholine and nicotine.

Pathways

The involvement of CHRNA3 in cholinergic signaling pathways is significant. It contributes to the role of nicotinic acetylcholine receptors in the addictive properties of substances like nicotine. Interaction with proteins such as CHRNB2 (nicotinic acetylcholine receptor beta-2 subunit) exemplifies how CHRNA3 participates in modulating synaptic strength and integration in the central nervous system. Its regulatory activity in these pathways demonstrates its importance in maintaining the balance of neurotransmitter systems.

Aberrations in CHRNA3 expression or function link to several neuropsychiatric and neurodegenerative conditions. It associates with nicotine dependence and lung cancer where alterations in receptor activity influence disease progression. In these contexts CHRNA3 interacts with proteins like CHRNB4 further implicating its role in pathological nicotine dependence and suggesting potential for targeted intervention strategies.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed : 31488329, PubMed : 31708116). CHRNA3 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, with CHRNA5, and CHRNB3 as accesory subunits (PubMed : 20881005, PubMed : 8663494). CHRNA3 : CHRNB4 being predominant in neurons of the autonomic ganglia, it is known as ganglionic nicotinic receptor (PubMed : 31488329). CHRNA3 : CHRNB4 or CHRNA3 : CHRNA5 : CHRNB4 play also an important role in the habenulo-interpeduncular tract, modulating the mesolimbic dopamine system and affecting reward circuits and addiction (By similarity). Hypothalamic CHRNA3 : CHRNB4 nAChR activation by nicotine leads to activation of POMC neurons and a decrease in food intake (By similarity). Also expressed in the urothelium where it modulates reflex bladder activity by increasing intracellular calcium through extracellular influx and basal ATP release (By similarity).

See full target information CHRNA3

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10]

Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (AB233717)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise