AB240004

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Recombinant Monoclonal Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

NACRA4, CHRNA4, Neuronal acetylcholine receptor subunit alpha-4

1 이미지

Unknown

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] - BSA and Azide free (AB240004)

This data was developed using ab124832, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (<a href='/ko/products/primary-antibodies/nicotinic-acetylcholine-receptor-alpha-4-chrna4-antibody-epr45632-ab124832'>ab124832</a>) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

A673 cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

Neuro-2a cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4563(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

제품 세부 정보

ab240004 is the carrier-free version of ab124832.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor alpha 4 subunit also known as CHRNA4 forms a part of the nicotinic cholinergic receptor family. It serves as a ligand-gated ion channel responsive to the neurotransmitter acetylcholine. This receptor's proper function involves the binding of acetylcholine which results in the opening of an ion channel leading to ion flow across the membrane. CHRNA4 has a molecular mass of about 67 kDa and typically pairs with other subunits like beta 2 (β2) to form a functional receptor. It is primarily expressed in the central nervous system particularly within the cerebral cortex hippocampus and basal forebrain regions.

Biological function summary

CHRNA4's role extends beyond the basic mechanism of ion channel opening. It engages in the modulation of synaptic activity and participates in the cognitive processes due to its localization in the brain. CHRNA4 usually forms a receptor complex with other types of alpha and beta subunits such as the alpha 3 and beta 2 subunits contributing to the diversity due to its different subunit composition. This variety of subunit combinations determines distinct pharmacological and kinetic properties of the resulting ion channels.

Pathways

The CHRNA4 subunit involved in the neurotransmission pathways integrates into the cholinergic signaling pathway. This pathway is essential for modulating various cognitive functions including learning and memory. The CHRNA4 subunit closely interacts with other proteins such as CHRNB2 as part of the receptor complex functioning in the cholinergic synaptic transmission. This interaction supports the involvement in key neurotransmitter systems that regulate mood arousal and cognitive function.

Aberrations in CHRNA4 function link to several neurological conditions. It associates strongly with autosomal dominant nocturnal frontal lobe epilepsy where mutations in CHRNA4 can lead to hypersynchronization of neuronal activity. Additionally alterations in this receptor are implicated in nicotine addiction where genetic variations in CHRNA4 influence vulnerability. These disorders illustrate the broader role of CHRNA4 within the brain's network emphasizing its connection to other cholinergic receptors and proteins such as CHRNB2 which also share involvement in these disease pathways.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed : 22361591, PubMed : 27698419, PubMed : 29720657, PubMed : 38454578). CHRNA4 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, as well as CHRNA5 and CHRNB3 as accesory subunits. Is the most abundant nAChR subtype expressed in the central nervous system (PubMed : 16835356, PubMed : 22361591, PubMed : 27698419, PubMed : 29720657, PubMed : 38454578). Found in two major stoichiometric forms,(CHRNA4)3 : (CHRNB2)2 and (CHRNA4)2 : (CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation (PubMed : 27698419, PubMed : 29720657, PubMed : 38454578). Involved in the modulation of calcium-dependent signaling pathways, influences the release of neurotransmitters, including dopamine, glutamate and GABA (By similarity).

See full target information CHRNA4

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell division 20:22 PubMed41039618

2025

FGF4 drives tumor progression in triple-negative breast cancer via IL6/STAT3-mediated macrophage M2 polarization and immune suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanhe Zhang,Shushan Zhang,Yuanyuan Han

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] - BSA and Azide free

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] - BSA and Azide free (AB240004)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise