AB131279

Anti-Nidogen 2/NID-2 antibody [EPR8697]

RabMAb
Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Nidogen 2/NID-2 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

대체 명칭 보기

Nidogen-2, NID-2, Osteonidogen, NID2

2 이미지

Unknown

Western blot - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

All lanes:

Western blot - Anti-Nidogen 2/NID-2 antibody [EPR8697] (ab131279) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

BxPC-3 lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal lung lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 151 kDa

false

OI-RD Scanning - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

OI-RD Scanning

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OI-RD Scanning - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8697

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

'Nidogen 2' also called 'NID-2' or '"nidogen protein'" or '"nid-2"' is a glycoprotein found in basement membranes. It has a molecular mass around 150 kDa and expresses across various tissues particularly in the vascular structures and certain epithelial tissues. This protein has sulfated and carbohydrate-rich regions making it important in forming stable extracellular matrix architecture.

Biological function summary

'nidogen 2' plays roles in maintaining the structural integrity of basement membranes. It interacts with other matrix components like laminin and collagen IV forming part of complexes that ensure the basal membrane's maintenance and stability. 'NID-2' provides structural support and helps secure cellular environments which is important for tissue repair and morphogenesis.

Pathways

'nidogen 2' contributes to the extracellular matrix remodeling and cell signaling pathways. It participates in the integrin signaling pathway where it interacts with proteins such as 'integrin' and 'perlecan'. These interactions help mediate cellular adhesion and signal transduction impacting cellular behavior like proliferation differentiation and migration processes.

Abnormalities in 'nidogen 2' relate to certain cancers and kidney disorders. In cancer disrupted interactions with 'laminin' may promote tumor cell invasion and metastasis. In kidney disorders such as nephrotic syndrome altered 'nidogen 2' function relates to basement membrane defects affecting kidney filtration function. Understanding its role could lead to insights into therapies for these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.

See full target information Nidogen-2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death discovery 9:159 PubMed37173310

2023

N-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangyang Qi,Chi Zhang,Huan Jian,Mengfan Hou,Yongfu Lou,Yi Kang,Wei Wang,Yigang Lv,Shenghui Shang,Chaoyu Wang,Xueying Li,Shiqing Feng,Hengxing Zhou

Nature communications 9:5150 PubMed30514914

2018

Spatially and functionally distinct subclasses of breast cancer-associated fibroblasts revealed by single cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Bartoschek,Nikolay Oskolkov,Matteo Bocci,John Lövrot,Christer Larsson,Mikael Sommarin,Chris D Madsen,David Lindgren,Gyula Pekar,Göran Karlsson,Markus Ringnér,Jonas Bergh,Åsa Björklund,Kristian Pietras

Bioscience, biotechnology, and biochemistry 80:1344-55 PubMed26966997

2016

Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Uenoyama,Ikuko Kakizaki,Aki Shiomi,Naoaki Saito,Yuko Hara,Taro Saito,Hisashi Ohnuki,Hiroko Kato,Ritsuo Takagi,Takeyasu Maeda,Kenji Izumi

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nidogen 2/NID-2 antibody [EPR8697]

Western blot - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

OI-RD Scanning - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise