AB248011

Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Niemann Pick C1 Like 1/NPC1L1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

NPC1-like intracellular cholesterol transporter 1, NPC1L1, Niemann-Pick C1-like protein 1

3 이미지

Unknown

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (AB248011)

This data was developed using ab124801, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (<a href='/ko/products/primary-antibodies/niemann-pick-c1-like-1-npc1l1-antibody-epr5717-ab124801'>ab124801</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysates at 10 µg

Lane 2:

HeLa lysates at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 149 kDa

false

Lab

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (AB248011)

This data was developed using ab124801, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 102 kDa,149 kDa,20 kDa,24 kDa,29 kDa,30 kDa,31 kDa,33 kDa,51 kDa,55 kDa,73 kDa,82 kDa

Observed band size: 102 kDa,149 kDa,20 kDa,24 kDa,29 kDa,42 kDa,51 kDa,65 kDa,73 kDa,75 kDa,78 kDa

false

Lab

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (AB248011)

This data was developed using ab124801, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 149 kDa,45 kDa

Observed band size: 149 kDa,45 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5717

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab248011 is the carrier-free version of ab124801.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Niemann Pick C1 Like 1 (NPC1L1) also known simply as NPC1L1 is a protein that plays a critical role in the intestinal absorption of dietary cholesterol. NPC1L1 is a multi-pass membrane protein located in the brush border of small intestine enterocytes and also present in the liver. It consists of approximately 1334 amino acids giving it a mass of around 148 kDa. This protein serves as a conduit for cholesterol as it moves across the cell membrane taking part in the cholesterol uptake mechanism.

Biological function summary

NPC1L1 is essential for maintaining cholesterol homeostasis within the body. It is not part of a larger protein complex but works closely with other proteins to facilitate lipid incorporation into chylomicrons. Inhibitors of NPC1L1 such as ezetimibe effectively reduce blood cholesterol levels by blocking this absorption route. The presence of NPC1L1 on the apical surface of enterocytes emphasizes its function in the body's cholesterol metabolism impacting systemic lipid levels.

Pathways

NPC1L1 is involved with cholesterol absorption pathways an important component of lipid homeostasis. This pathway involves other important proteins such as the Niemann-Pick C1 protein (NPC1) and ATP-binding cassette transporters like ABCG5 and ABCG8. These proteins along with NPC1L1 coordinate the regulation of cholesterol levels by balancing absorption and excretion aiding in maintaining overall homeostasis in the body.

NPC1L1's role in cholesterol regulation links it directly to atherosclerosis and hypercholesterolemia. Elevated levels of cholesterol can lead to plaque formation in arteries contributing to coronary artery disease. Ezetimibe a drug targeting NPC1L1 demonstrates this connection as it helps lower cholesterol levels by inhibiting NPC1L1 function. Moreover interactions with other proteins like APOB (Apolipoprotein B) and LDL receptor exemplify the broader implication of NPC1L1 in lipid metabolism disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a major role in cholesterol homeostasis (PubMed : 22095670). Critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte (PubMed : 22095670). Involved in plant sterol absorption, it transports sitosterol, although at lower rates than cholesterol (By similarity). Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorption and is approved for the treatment of hypercholesterolemia (PubMed : 15928087). May have a function in the transport of multiple lipids and their homeostasis, thereby influencing lipid metabolism regulation (PubMed : 15671032). May be involved in caveolin trafficking from the plasma membrane (By similarity). In addition, acts as a negative regulator of NPC2 and down-regulates its expression and secretion by inhibiting its maturation and accelerating its degradation (PubMed : 22095670).

See full target information NPC1-like intracellular cholesterol transporter 1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of virology 97:e0190722 PubMed36946735

2023

Characterization of the Impact of Merkel Cell Polyomavirus-Induced Interferon Signaling on Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Wang,June F Yang,Taylor E Senay,Wei Liu,Jianxin You

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (AB248011)

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (AB248011)

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free (AB248011)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise