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AB325231

Anti-Nipah Virus Glycoprotein F antibody [CG11]

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Mouse Recombinant Monoclonal TLS/FUS antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Nipah virus, Nipah virus samples.
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Indirect ELISA - Anti-Nipah Virus Glycoprotein F antibody [CG11] (AB325231)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Nipah Virus Glycoprotein F antibody [CG11] (AB325231)

Indirect ELISA analysis of ab325231 at 2000-0 ng/ml. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Mouse IgG, F(ab')₂ fragment specific at 1 : 20000 dilution dilution.

Antigen : Nipah Virus Glycoprotein F;Nipah Virus Glycoprotein G.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Nipah Virus Glycoprotein F antibody [CG11] (AB325231)
  • WB

Supplier Data

Western blot - Anti-Nipah Virus Glycoprotein F antibody [CG11] (AB325231)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Nipah Virus Glycoprotein F antibody [CG11] (ab325231) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with Nipah virus Fusion glycoprotein F0 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with Hendra virus (isolate Horse/Autralia/Hendra/1994) Fusion glycoprotein F0 expression vector containing a myc-His-tag® whole cell lysate at 2 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 8s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CG11

Isotype

IgG1

Carrier free

No

Reacts with

Nipah virus

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody does cross-react with Hendra virus Glycoprotein F.</p>

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보완 제품

Primary Antibodies

AB183497

Anti-VSV-G tag antibody [EPR12997]

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Western blot - Anti-VSV-G tag antibody [EPR12997] (AB183497)

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Primary Antibodies

AB271180

Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein

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Flow Cytometry (Intracellular) - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (AB271180)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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  • 24
Primary Antibodies

AB313343

Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] (AB313343)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Nipah virus": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "500 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Nipah virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states : pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (F protein) probably interacts with G at the virion surface. Upon G binding to its cellular receptor, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between cell and virion membranes. Later in infection, F proteins expressed at the plasma membrane of infected cells could mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis.
See full target information Fusion glycoprotein F0

대체 명칭 보기

Fusion glycoprotein F0, Protein F, F
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com