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AB61705

Anti-NK-1R antibody

1

(1 리뷰)

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(4 제품이 사용된 논문 )

Goat Polyclonal NK-1R antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TACR1 aa 200 to C-terminus.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-NK-1R antibody (AB61705)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NK-1R antibody (AB61705)

Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized Neuro2a (Mouse neuroblastoma cell line) cells labelling NK-1R with primary Anti-NK-1R antibody (ab61705) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml.

Western blot - Anti-NK-1R antibody (AB61705)
  • WB

Supplier Data

Western blot - Anti-NK-1R antibody (AB61705)

Lane 1-2 : ab61705 (1µg/ml) staining of HeLa (A) and (0.5ug/ml) Neuro2a (B) cell lysate. Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.

All lanes:

Western blot - Anti-NK-1R antibody (ab61705)

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 35 µg

Lane 2:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 35 µg

Predicted band size: 46 kDa

Observed band size: 48 kDa

false

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TACR1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25103

Specificity

This antibody is expected to recognise both reported isoforms (NP_001049.1 and NP_056542.1).

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대체품

Primary Antibodies

AB219600

Anti-NK-1R antibody - Extracellular domain

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NK-1R antibody - Extracellular domain (AB219600)

  • 0
  • 0

보완 제품

Primary Antibodies

AB14184

Anti-Substance P antibody [SP-DE4-21]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)

  • 4
  • 15
Primary Antibodies

AB16046

Anti-FOXP2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody (AB16046)

  • 5
  • 20
Primary Antibodies

AB305299

Anti-TRPV1 antibody [EPR25057-67]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [EPR25057-67] (AB305299)

  • 3
  • 1
Primary Antibodies

AB108986

Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (AB108986)

  • 5
  • 12
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Receptor for the tachykinin substance P, also able to bind and respond to tachynins neurokinin A/substance K and neurokinin B/neuromedin-K (PubMed : 1718267, PubMed : 15452552). The rank order of affinity of this receptor to tachykinins is : substance P > neurokinin A/substance K > neurokinin B/neuromedin-K (PubMed : 1718267). Substance P binding to its receptor triggers G protein-coupled receptor signaling via activation of phosphatidylinositol hydrolysis by phospholipase C. Substance P binding also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP) (By similarity).
See full target information TACR1

대체 명칭 보기

NK1R, TAC1R, TACR1, Substance-P receptor, SPR, NK-1 receptor, Tachykinin receptor 1, NK-1R
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Stem cell reports 17:849-863 PubMed35334220

2022

Substance P/neurokinin-1 receptor pathway blockade ameliorates limbal stem cell deficiency by modulating mTOR pathway and preventing cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Lasagni Vitar,Francesca Triani,Marco Barbariga,Philippe Fonteyne,Paolo Rama,Giulio Ferrari

Investigative ophthalmology & visual science 62:21 PubMed34698773

2021

A Hypothalamic-Controlled Neural Reflex Promotes Corneal Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Mayra Lasagni Vitar,Philippe Fonteyne,Linda Chaabane,Paolo Rama,Giulio Ferrari

Investigative ophthalmology & visual science 62:26 PubMed33729475

2021

Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Mayra Lasagni Vitar,Marco Barbariga,Philippe Fonteyne,Fabio Bignami,Paolo Rama,Giulio Ferrari

Artificial cells, nanomedicine, and biotechnology 47:1628-1634 PubMed31010320

2019

Antagonism of NK-1R using aprepitant suppresses inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Liu,Yuelan Zhu,Wei Zheng,Tangliang Qian,Haiyu Wang,Xiujuan Hou
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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