AB230900

Anti-NKG2C antibody - N-terminal

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Rabbit Polyclonal NKG2C antibody. N-terminal. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KLRC2 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

CD159c, NKG2C, KLRC2, NKG2-C type II integral membrane protein, CD159 antigen-like family member C, NK cell receptor C, NKG2-C-activating NK receptor

3 이미지

Flow Cytometry (Intracellular) - Anti-NKG2C antibody - N-terminal (AB230900)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NKG2C antibody - N-terminal (AB230900)

Intracellular flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling NKG2C with ab230900 at 1/10 dilution (green) compared with a negative control (blue).

FITC-conjugated goat-anti-rabbit secondary antibodies were used.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2C antibody - N-terminal (AB230900)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2C antibody - N-terminal (AB230900)

Formalin-fixed, paraffin-embedded human colon tissue stained for NKG2C with ab230900 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-NKG2C antibody - N-terminal (AB230900)

All lanes:

Western blot - Anti-NKG2C antibody - N-terminal (ab230900) at 1/1000 dilution

All lanes:

HL-60 (human promyelocytic leukemia cell line) cell lysate at 35 µg

Predicted band size: 26 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human KLRC2 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P26717

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NKG2C also known as CD159c is a receptor protein that plays an important role in immune responses. This protein has an approximate mass of 30 kDa and is usually found on the surface of natural killer (NK) cells. NKG2C belongs to the family of C-type lectin receptors and forms heterodimers with the signaling adaptor protein DAP12. These heterodimers are expressed on NK cells and certain T cell subsets enabling the cells to recognize and respond to infected or transformed cells.

Biological function summary

NKG2C serves as an activating receptor for NK cells and certain T cells enhancing their cytotoxic functions. It participates in the recognition of cells expressing human cytomegalovirus (HCMV) proteins allowing the immune system to target and eliminate these infected cells efficiently. This receptor acts in a complex with DAP12 through which it transmits activation signals inside the immune cells. The engagement of NKG2C with its ligands initiates the production and release of cytokines and lytic granules promoting the destruction of target cells.

Pathways

NKG2C functions within the immune signaling pathways by interacting with other receptors and molecules on NK cells. The receptor is involved in pathways that activate NK cell-mediated cytotoxicity and cytokine production. NKG2C interacts with other natural killer receptors such as NKG2A and NKG2E contributing to the regulation of immune responses. The balance of signals from different receptors including NKG2C and NKG2A defines the activation and inhibition states of NK cells impacting both innate and adaptive immunity.

NKG2C expression has significance in viral infections and autoimmune diseases. In cases of viral infections like HCMV increased expression of NKG2C enhances the immune response against the virus by recognizing the virus-infected cells. In autoimmune disorders changes in NKG2C expression or function can alter immune regulation which may exacerbate disease conditions. NKG2C and its relationship with DAP12 as well as other immune receptors make it a valuable target for therapeutic interventions in these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Immune activating receptor involved in self-nonself discrimination. In complex with KLRD1 on cytotoxic lymphocyte subsets, recognizes non-classical major histocompatibility (MHC) class Ib HLA-E loaded with signal sequence-derived peptides from non-classical MHC class Ib HLA-G molecules, likely playing a role in the generation and effector functions of adaptive natural killer (NK) cells and in maternal-fetal tolerance during pregnancy (PubMed : 30134159, PubMed : 37264229, PubMed : 9754572). Regulates the effector functions of terminally differentiated cytotoxic lymphocyte subsets, and in particular may play a role in adaptive NK cell response to viral infection (PubMed : 20952657, PubMed : 21825173). Upon HLA-E-peptide binding, transmits intracellular signals via the adapter protein TYROBP/DAP12, triggering the phosphorylation of proximal signaling molecules and cell activation (PubMed : 15940674, PubMed : 9655483).

See full target information KLRC2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature immunology 26:279-293 PubMed39779871

2025

A multi-kinase inhibitor screen identifies inhibitors preserving stem-cell-like chimeric antigen receptor T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Song,Ourania Tsahouridis,Simone Stucchi,Tara Walhart,Sophie Mendell,P Brian Hardy,Matthew Axtman,Shiva K R Guduru,Thomas S K Gilbert,Lee M Graves,Laura E Herring,Barbara Savoldo,Xingcong Ma,Mark Woodcock,Justin J Milner,Anastasia Ivanova,Kenneth H Pearce,Yang Xu,Gianpietro Dotti

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NKG2C antibody - N-terminal

Flow Cytometry (Intracellular) - Anti-NKG2C antibody - N-terminal (AB230900)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2C antibody - N-terminal (AB230900)

Western blot - Anti-NKG2C antibody - N-terminal (AB230900)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise