AB119802

Anti-NLN antibody [OTI1D6]

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(3 제품이 사용된 논문 )

Mouse Monoclonal NLN antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Transfected cell lysate - Human, Human, African green monkey, Transfected cell line - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NLN.

대체 명칭 보기

AGTBP, KIAA1226, NLN, Angiotensin-binding protein, Microsomal endopeptidase, Mitochondrial oligopeptidase M, Neurotensin endopeptidase, MEP

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Paraffin-embedded human tonsil tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Paraffin-embedded human pancreas carcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Paraffin-embedded human bladder carcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-NLN antibody [OTI1D6] (AB119802)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NLN antibody [OTI1D6] (AB119802)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab119802 at 1/100 dilution stained for NLN (Red) compared to a nonspecific negetive control antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (AB119802)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (AB119802)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY NLN, stained for NLN (green) using ab119802 at 1/100 dilution in ICC/IF.

Unknown

Western blot - Anti-NLN antibody [OTI1D6] (AB119802)

All lanes:

Western blot - Anti-NLN antibody [OTI1D6] (ab119802) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg

Lane 7:

MDCK cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 35 µg

Predicted band size: 81 kDa

false

Unknown

Western blot - Anti-NLN antibody [OTI1D6] (AB119802)

All lanes:

Western blot - Anti-NLN antibody [OTI1D6] (ab119802) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T cell lysate transfected with pCMV6-ENTRY NLN cDNA at 5 µg

Predicted band size: 81 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1D6

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human, African green monkey

Applications

WB, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NLN.

Q9BYT8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

The clone number has been updated from 1D6 to OTI1D6, both clone numbers name the same clone.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant by affinity chromatography.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NLN also known as Neurolysin is a 77 kDa metalloendopeptidase enzyme with an ability to cleave neuropeptides at specific sites. Researchers frequently observe NLN in the central nervous system kidneys testis and muscle tissues. This enzyme notably exists as a mitochondrial matrix protein showing an evolutionary conservation in its structure across mammalian species. NLN plays a role in processing bioactive peptides displaying similarity in substrate specificity to its better-known counterpart thimet oligopeptidase.

Biological function summary

NLN degrades neuropeptides which aids in regulating their activity and maintaining homeostasis. It does not typically form part of a large complex but interacts directly with peptide substrates to exert its effects. By modulating peptide availability NLN influences neurological functions and potentially affects signaling pathways involved in mood pain and energy regulation. Researchers continue to explore NLN's physiological roles and interactions though its exact contributions outside peptide processing remain less detailed.

Pathways

Studies show NLN engages in the metabolism of bioactive peptides including enkephalins and tachykinins. In these pathways NLN acts to terminate peptide signaling by cleaving them which alters receptor interactions. Within these mechanisms NLN shares activity with proteins like neprilysin both contributing to peptide degradation but acting on different substrates or within distinct cellular locales. These pathways are critical for maintaining neural function and homeostasis underlying many processes in the nervous system.

Dysregulation of NLN activity links to conditions such as hypertension and Alzheimer's disease. In hypertension improper peptide degradation can affect blood pressure regulation. In Alzheimer’s disease NLN’s inability to regulate amyloid-beta peptides in association with neprilysin contributes to plaque accumulation a hallmark of the disease. Understanding NLN’s role offers potential therapeutic insights for correcting peptide imbalances related to these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A (By similarity). Acts as a regulator of cannabinoid signaling pathway by mediating degradation of hemopressin, an antagonist peptide of the cannabinoid receptor CNR1 (By similarity).

See full target information NLN

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

NPJ Parkinson's disease 10:120 PubMed38906862

2024

Enhancing mitochondrial proteolysis alleviates alpha-synuclein-mediated cellular toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Zhang,Linhao Ruan,Hu Wang,Jin Zhu,Taibo Li,Gordon Sun,Yi Dong,Yuhao Wang,Gil Berreby,Ashley Shay,Rong Chen,Sreekumar Ramachandran,Valina L Dawson,Ted M Dawson,Rong Li

Science translational medicine 12: PubMed32269163

2020

The mitochondrial peptidase, neurolysin, regulates respiratory chain supercomplex formation and is necessary for AML viability.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Mirali,Aaron Botham,Veronique Voisin,Changjiang Xu,Jonathan St-Germain,David Sharon,Fieke W Hoff,Yihua Qiu,Rose Hurren,Marcela Gronda,Yulia Jitkova,Boaz Nachmias,Neil MacLean,Xiaoming Wang,Andrea Arruda,Mark D Minden,Terzah M Horton,Steven M Kornblau,Steven M Chan,Gary D Bader,Brian Raught,Aaron D Schimmer

Scientific reports 6:28176 PubMed27307030

2016

Arm Selection Preference of MicroRNA-193a Varies in Breast Cancer.

Applications

Species

Unspecified reactive species

Kuo-Wang Tsai,Chung-Man Leung,Yi-Hao Lo,Ting-Wen Chen,Wen-Ching Chan,Shou-Yu Yu,Ya-Ting Tu,Hing-Chung Lam,Sung-Chou Li,Luo-Ping Ger,Wen-Shan Liu,Hong-Tai Chang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NLN antibody [OTI1D6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Flow Cytometry (Intracellular) - Anti-NLN antibody [OTI1D6] (AB119802)

Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (AB119802)

Western blot - Anti-NLN antibody [OTI1D6] (AB119802)

Western blot - Anti-NLN antibody [OTI1D6] (AB119802)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise