Anti-NMDAR1 antibody [N308/48] - Neuronal Marker

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [N308/48] - Neuronal Marker (AB193310)

IHC image of NMDAR1 staining in Human Normal Cerebral Cortex formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab193310, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

Lab

Western blot - Anti-NMDAR1 antibody [N308/48] - Neuronal Marker (AB193310)

Lanes 1-5 were blocked with 2% BSA.

Lanes 6-10 were blocked with 3% milk.

We recommend using 3% milk as the blocking agent for Western blot.

All lanes:

Anti-NMDAR1 antibody [N308/48] (ab193310) at 1 µg/mL

Lanes 1 and 6:

Brain (Mouse) Tissue Lysate at 20 µg

Lanes 2 and 7:

Brain (Rat) Tissue Lysate at 20 µg

Lanes 3 and 8:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 20 µg

Lanes 4 and 9:

Spinal Cord (Mouse) Tissue Lysate at 20 µg

Lanes 5 and 10:

Spinal Cord (Rat) Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 105 kDa

Observed band size: 78 kDa

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Exposure time: 12min