Anti-Noggin antibody [EPR26109-174]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Noggin antibody [EPR26109-174] (AB326564)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A-172 (human brain glioblastoma cell) untreated and A-172 treated with 300 ng/ml BFA for 24 hours cells labelling Noggin with ab326564 at 1/50 (10.52 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing increased cytoplasmic staining in A-172 cells (shown in green) treated with 300 ng/ml BFA (Brefeldin A) for 24 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• WB

Lab

Western blot - Anti-Noggin antibody [EPR26109-174] (AB326564)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HepG2.

The identity of the higher MW band at approximately 37 kDa (in lane 3) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Noggin antibody [EPR26109-174] (ab326564) at 1/1000 dilution

Lane 1:

Untreated A-172 (human brain glioblastoma cell) whole cell lysate at 50 µg

Lane 2:

A-172 treated with 300ng/ml BFA for 24h whole cell lysate at 50 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 50 µg

Lane 4:

Untreated RD (human muscle multinucleated spindle-shaped cell) whole cell lysate at 50 µg

Lane 5:

RD treated with 300ng/ml BFA for 24h whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 25 kDa,36 kDa

false

Exposure time: 59s