AB242201

Anti-NOL5A antibody [CL2603]

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Mouse Monoclonal NOL5A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NOP56 aa 450-550.

대체 명칭 보기

NOL5A, NOP56, Nucleolar protein 56, Nucleolar protein 5A

13 이미지

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma cell line) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human liver tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human kidney tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human cervix tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of U-251 MG (Human brain glioma cell line) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling NOL5A using ab242201 at 4 μg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

Supplier Data

Western blot - Anti-NOL5A antibody [CL2603] (AB242201)

Loading control : anti-GAPDH.

All lanes:

Western blot - Anti-NOL5A antibody [CL2603] (ab242201) at 1 µg/mL

Lane 1:

Non-transfected U-251 MG (Human brain glioma cell line) cell lysate

Lane 2:

siRNA#1 transfected U-251 MG cell lysate

Lane 3:

siRNA#2 transfected U-251 MG cell lysate

Predicted band size: 66 kDa

false

Supplier Data

Western blot - Anti-NOL5A antibody [CL2603] (AB242201)

All lanes:

Western blot - Anti-NOL5A antibody [CL2603] (ab242201) at 1 µg/mL

All lanes:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 66 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2603

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NOP56 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

O00567

Epitope

Binds to an epitope located within the peptide sequence FSKEELMSSDLEETA as determined by overlapping synthetic peptides.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NOL5A also known as Nucleolar Protein 5A or hNop56 functions as a nucleolar protein involved in ribosome biogenesis. It is characterized by a mass of approximately 63 kDa. NOL5A exhibits high expression within the nucleolus of cells where it participates in the assembly of ribosomal subunits. Specifically NOL5A plays a role in the modification and maturation of pre-ribosomal RNA. Its expression is observed in rapidly dividing cells highlighting its importance in cellular growth and proliferation.

Biological function summary

Ribosome biogenesis depends on several proteins and NOL5A serves as a core component in the small nucleolar ribonucleoprotein (snoRNP) complex. This complex is responsible for the processing and chemical modification of small nucleolar RNAs (snoRNAs) which are essential for the production of functional ribosomes. NOL5A helps facilitate the cleavage and assembly steps necessary for pre-rRNA to mature into functional rRNAn important for effective protein synthesis within the cell. Its activity is important to maintaining proper ribosome function ensuring efficient protein translation.

Pathways

NOL5A acts within the rRNA processing pathway and is also linked to the mTOR signaling pathway. In the rRNA processing pathway NOL5A's role in the nucleoplasm involves cooperation with other nucleolar proteins like fibrillarin and Nop58 to orchestrate rRNA processing. In the mTOR pathway the regulation of ribosome production connects NOL5A to protein synthesis pathways impacting cell growth and proliferation. mTOR signaling influences many cellular processes and NOL5A’s function in ribosome biogenesis links it to these critical growth and survival pathways.

NOL5A has connections with cancer and ribosomopathy. Altered expression of NOL5A has implications in various cancers as its role in ribosome production can drive aberrant cell proliferation. Additionally proper function of NOL5A is essential in preventing ribosomopathies disorders characterized by defects in ribosome biogenesis. These disorders often result in bone marrow failure or craniofacial abnormalities. Studies investigating NOL5A's interactions with proteins such as NPM1 show its involvement in nucleolar stress responses which can influence cancer progression and response to therapy.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in the early to middle stages of 60S ribosomal subunit biogenesis. Required for the biogenesis of box C/D snoRNAs such U3, U8 and U14 snoRNAs (PubMed : 12777385, PubMed : 15574333). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that function in methylation of multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs) (PubMed : 12777385, PubMed : 39570315).

See full target information NOP56

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 23:84 PubMed35126726

2022

Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Mao,Jun Wang,Fen Luo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOL5A antibody [CL2603]

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (AB242201)

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (AB242201)

Western blot - Anti-NOL5A antibody [CL2603] (AB242201)

Western blot - Anti-NOL5A antibody [CL2603] (AB242201)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise