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AB307838

Anti-NPAT antibody [27/NPAT] - BSA and Azide free

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Mouse Recombinant Monoclonal NPAT antibody. Carrier free. Suitable for ICC/IF and reacts with Human samples.

대체 명칭 보기

CAND3, E14, NPAT, Protein NPAT, Nuclear protein of the ataxia telangiectasia mutated locus, p220, Nuclear protein of the ATM locus

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] - BSA and Azide free (AB307838)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] - BSA and Azide free (AB307838)

This image was produced using ab307837, the same clone but in a different formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized Saos-2 (human osteosarcoma epithelial cell) cells labelling NPAT with ab307837 at 1/250 dilution (3.99 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing nuclear foci staining in Saos-2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] - BSA and Azide free (AB307838)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] - BSA and Azide free (AB307838)

This image was produced using ab307837, the same clone but in a different formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling NPAT with ab307837 at 1/250 dilution (3.99 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2ug/ml) (Green). Confocal image showing nuclear foci staining in U-2 OS cell line. The number of positive-staining cells decreased after treatment with 20 uM roscovitine for 48 h (PMID : 14976556). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (5ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2ug/ml).

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] - BSA and Azide free (AB307838)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] - BSA and Azide free (AB307838)

This image was produced using ab307837, the same clone but in a different formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling NPAT with ab307837 at 1/250 dilution (3.99 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2ug/ml) (Green). Confocal image showing nuclear foci staining in A431 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (10ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2ug/ml).

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

27/NPAT

Isotype

IgG2b

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB11826

Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

  • 4
  • 16
Primary Antibodies

AB31830

Anti-Histone H4 antibody [mAbcam 31830] - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H4 antibody [mAbcam 31830] - ChIP Grade (AB31830)

  • 5
  • 6
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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제품 세부 정보

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NPAT also known as nuclear protein ataxia-telangiectasia locus is a protein that weighs approximately 220 kDa. It is widely present in various tissues with a high expression in proliferating cells. NPAT participates in cell cycle regulation and links directly to the control of histone gene expression during the G1/S phase transition. Its presence in the nuclear matrix further highlights its integrative role in gene regulation.
Biological function summary

NPAT plays an important role in the cell cycle's advancement by associating with the MMB (Myb-MuvB) complex. This interaction signifies its importance in DNA replication and cell cycle progression. NPAT activates transcription upon phosphorylation by CDK2/cyclin E influencing S-phase entry. It acts as a transcriptional regulator for histone gene expression necessary for chromatin structure and genome stability.

Pathways

NPAT integrates into the cyclin/CDK regulatory pathway governing cell cycle progression and is related to histone gene clusters regulation. The interaction with CDK2 illustrates its part in the regulation of cell proliferation. NPAT also associates with the E2F cell cycle pathway further influencing gene transcription important for DNA replication.

NPAT relates to cancer and ataxia-telangiectasia. These connections highlight its importance in cell cycle control disruption which can lead to oncogenesis. NPAT's relationship with the ATM protein emerges in the context of ataxia-telangiectasia suggesting a link between DNA damage response defects and neurological disorders. These associations reinforce NPAT’s involvement in maintaining cellular health and genomic integrity.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription regulator required for progression through the G1 and S phases of the cell cycle and for S phase entry (PubMed : 12665581, PubMed : 15555599, PubMed : 9472014). Acts as a key transcription regulator of histones (PubMed : 10995386, PubMed : 10995387, PubMed : 12724424, PubMed : 14585971, PubMed : 14612403, PubMed : 15988025, PubMed : 16131487, PubMed : 17163457, PubMed : 25339177, PubMed : 40516528, PubMed : 40516529). Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with GON4L and MIZF (PubMed : 10995386, PubMed : 10995387, PubMed : 12724424, PubMed : 14585971, PubMed : 14612403, PubMed : 15988025, PubMed : 16131487, PubMed : 17163457, PubMed : 25339177). Together with CRAMP1, binds to the promoters of H1 genes (H1-2, H1-3, H1-4, H1-5 and H1-10/H1x), driving their transcription (PubMed : 40516528, PubMed : 40516529). Also positively regulates the ATM, MIZF and PRKDC promoters (PubMed : 17826007, PubMed : 17974976, PubMed : 8743993, PubMed : 8923007). Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters (PubMed : 17967892).
See full target information NPAT
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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