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AB88691

Anti-NPRL2 antibody

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(2 제품이 사용된 논문 )

Mouse Polyclonal NPRL2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NPRL2.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-NPRL2 antibody (AB88691)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NPRL2 antibody (AB88691)

ab88691, at 10 μg/ml, staining NPRL2 in HeLa cell by Immunofluorescence.

Western blot - Anti-NPRL2 antibody (AB88691)
  • WB

Unknown

Western blot - Anti-NPRL2 antibody (AB88691)

All lanes:

Western blot - Anti-NPRL2 antibody (ab88691) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 44 kDa

Observed band size: 44 kDa

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주요 정보

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NPRL2.

Q8WTW4

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보완 제품

Primary Antibodies

AB213181

Anti-DEPDC5 antibody [EPR20497-23]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-DEPDC5 antibody [EPR20497-23] (AB213181)

  • 5
  • 2
Primary Antibodies

AB124885

Anti-FLCN antibody [EPNCIR147]

RabMAb|Recombinant|KO Validated

Western blot - Anti-FLCN antibody [EPNCIR147] (AB124885)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB88686

Anti-ORC6L antibody

Immunocytochemistry/ Immunofluorescence - Anti-ORC6L antibody (AB88686)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NPRL2 also known as Nitrogen Permease Regulator-Like 2 plays a role in regulating the mTORC1 pathway. It is known to form part of the GATOR1 complex acting as a negative regulator of the mTORC1 signaling. NPRL2 has a molecular mass of approximately 41 kDa. This protein shows expression in various tissues with notable expression in the brain and lung.
Biological function summary

NPRL2 and its role in the GATOR1 complex importantly manages cellular response to nutrient availability and growth signals. The GATOR1 complex comprises NPRL2 NPRL3 and DEPDC5 which collectively suppress mTORC1 activity under nutrient-poor conditions. This suppression promotes autophagy and inhibits protein synthesis affecting how cells adapt to nutritional stress.

Pathways

NPRL2 is integral to the amino acid sensing branch of the mTORC1 signaling pathway. It plays a part in the nutrient sensing pathway alongside the proteins NPRL3 and DEPDC5. The mTORC1 pathway essential for controlling cell growth and metabolism depends on the regulated activity of NPRL2 and its partners to respond accurately to metabolic cues.

Altered NPRL2 function links to cancer and epilepsy. Mutations in the GATOR1 complex affecting proteins like DEPDC5 and NPRL3 in conjunction with NPRL2 have associations with tumor growth due to disrupted mTORC1 regulation. In epilepsy especially the focal cortical dysplasia type NPRL2 mutations lead to imbalanced mTORC1 activity contributing to the development of seizures.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalytic component of the GATOR1 complex, a multiprotein complex that functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway (PubMed : 23723238, PubMed : 29590090, PubMed : 35338845, PubMed : 38006878). In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling (PubMed : 23723238, PubMed : 29590090, PubMed : 35338845). In the presence of abundant amino acids, the GATOR1 complex is ubiquitinated and inhibited by GATOR2 (PubMed : 23723238, PubMed : 36528027). Within the GATOR1 complex, NPRL2 constitutes the catalytic subunit that mediates the GTPase activator activity and under methionine-sufficient conditions, the GTPase activator activity is inhibited by PRMT1 through methylation and consequently inducing timely mTORC1 activation (PubMed : 27173016, PubMed : 30651352, PubMed : 35338845).. Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling (PubMed : 18616680). Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at 'Tyr-9', 'Tyr-373' and 'Tyr-376' residues (PubMed : 18616680). May act as a tumor suppressor (PubMed : 18616680). Suppresses cell growth and enhances sensitivity to various anticancer drugs (PubMed : 18616680).
See full target information NPRL2

대체 명칭 보기

TUSC4, NPRL2, GATOR1 complex protein NPRL2, Gene 21 protein, Nitrogen permease regulator 2-like protein, Tumor suppressor candidate 4, G21 protein, NPR2-like protein
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Medical science monitor : international medical jo 24:1424-1433 PubMed29519997

2018

Overexpression of Nitrogen Permease Regulator Like-2 (NPRL2) Enhances Sensitivity to Irinotecan (CPT-11) in Colon Cancer Cells by Activating the DNA Damage Checkpoint Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Liu,Bingrong Liu

Cancer biology & therapy 15:489-95 PubMed24521741

2014

Relationship between tumor and peripheral blood NPRL2 mRNA levels in patients with colorectal adenoma and colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ai-yun Liu,Dian-Ggang Liu,Ya-ju Du,Feng-hua Pei,Guang Yang,Bing-rong Liu,Hui-tao Zhang,Xin-hong Wang,Yu-jing Fan,Ying-zhun Chen,Yang Jiang,Jing Chen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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