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AB122427

Anti-NRAP antibody

4

(1 리뷰)

|

(2 제품이 사용된 논문 )

Rabbit Polyclonal NRAP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NRAP.

대체 명칭 보기

Nebulin-related-anchoring protein, N-RAP, NRAP

4 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (AB122427)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (AB122427)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart muscle tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (AB122427)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (AB122427)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (AB122427)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (AB122427)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (AB122427)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (AB122427)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NRAP.

Q86VF7

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보완 제품

Primary Antibodies

AB52862

Anti-Cardiac Troponin I antibody [EP1106Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Cardiac Troponin I antibody [EP1106Y] (AB52862)

  • 5
  • 2
Primary Antibodies

AB154263

Anti-ARHGEF3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF3 antibody (AB154263)

  • 0
  • 0
Primary Antibodies

AB68167

Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (AB68167)

  • 5
  • 9
Primary Antibodies

AB203515

Anti-TNNI1 antibody [EPR17120-11]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] (AB203515)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The NRAP protein also known as Nebulin-Related Anchoring Protein serves as an essential component of muscle sarcomeres. It functions mechanically by anchoring nebulin filaments to the sarcomeric Z-lines contributing to the integrity of muscle fiber structure. The molecular weight of NRAP is approximately 197 kDa. This protein expresses highly in skeletal and cardiac muscles playing an important role in maintaining muscle architecture and function in these tissues.
Biological function summary

NRAP participates in muscle development and repair. It forms part of a protein complex that includes nebulin and actin filaments which collectively manage the assembly and stabilization of sarcomeres. Through its interactions with these filaments NRAP regulates the length of thin filaments during myofibrillogenesis. This protein facilitates effective muscle contraction and regeneration by maintaining the sarcomeric structure.

Pathways

NRAP integrates into significant muscle-related pathways such as the actin cytoskeleton signaling pathway and the sarcomere organization pathway. It interacts with proteins like talin and vinculin within these pathways which are fundamental for cytoskeletal stability and muscle cell adherence. These interactions help coordinate the structural assembly of sarcomeres ensuring proper muscle functionality and response to mechanical stress.

Alterations in NRAP expression or function have associations with certain muscle-related conditions including cardiomyopathy and muscular dystrophy. In the context of cardiomyopathy NRAP interacts with proteins like myopalladin a factor in cardiac myofibril integrity. In muscular dystrophy mutations affecting NRAP can lead to defects in muscle fiber formation and repair which further underline its connection to muscle disease pathophysiology.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.
See full target information NRAP
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Skeletal muscle 10:2 PubMed31992366

2020

Expressing a Z-disk nebulin fragment in nebulin-deficient mouse muscle: effects on muscle structure and function.

Applications

Unspecified application

Species

Unspecified reactive species

Frank Li,Justin Kolb,Julie Crudele,Paola Tonino,Zaynab Hourani,John E Smith,Jeffrey S Chamberlain,Henk Granzier

Scientific reports 7:3362 PubMed28611399

2017

Homozygous truncating mutation in NRAP gene identified by whole exome sequencing in a patient with dilated cardiomyopathy.

Applications

WB

Species

Human

Grażyna T Truszkowska,Zofia T Bilińska,Angelika Muchowicz,Agnieszka Pollak,Anna Biernacka,Katarzyna Kozar-Kamińska,Piotr Stawiński,Piotr Gasperowicz,Joanna Kosińska,Tomasz Zieliński,Rafał Płoski
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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