AB34682

Anti-NRF1 antibody

Name: Anti-NRF1 antibody
Brand: Abcam Limited
SKU: AB34682
Rating: 3 (3 reviews)

(3 리뷰들)

(55 제품이 사용된 논문 )

Rabbit Polyclonal NRF1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 55 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Nrf1.

4 이미지

Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody (AB34682)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody (AB34682)

ICC/IF image of ab34682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody (AB34682)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody (AB34682)

Purified anti-NRF1 antibody (ab34682) was used at 5µg/ml to detect NRF1 in human testis. Nuclear staining in lymphocytes can be observed.

Unknown

Western blot - Anti-NRF1 antibody (AB34682)

All lanes:

Western blot - Anti-NRF1 antibody (ab34682) at 1/500 dilution

Lane 1:

HeLa Nuclear extract

Lane 2:

HeLa Whole Cell lysate

Secondary

All lanes:

IRDyeTM700 labelled goat-a-rabbit IgG at 1/2500 dilution

Predicted band size: 54 kDa

Observed band size: 67 kDa

false

AbReview46704****

Western blot - Anti-NRF1 antibody (AB34682)

All lanes:

Western blot - Anti-NRF1 antibody (ab34682) at 1/1000 dilution

Lane 1:

Mouse liver whole tissue lysate at 25 µg

Lane 2:

HeLa whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 54 kDa

Observed band size: 55 kDa,67 kDa

true

Exposure time: 50s

This image is courtesy of an anonymous Abreview

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Nrf1.

Q9WU00

Specificity

This protein A purified antibody is directed against mouse NRF1. BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat, chicken, frog and zebrafish based on very high sequence homology with the immunogen sequence. Cross reactivity with NRF1 homologues from other sources has not been determined.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "Splice variants exist for this protein that may result in the detection of lower molecular weight bands.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

This protein A purified antibody is directed against mouse NRF1. The product was purified from monospecific antiserum by protein A affinity purification.

보관 버퍼

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The nuclear respiratory factor 1 (NRF1) also known as Alpha-Pal is a transcription factor with an approximate molecular weight of 68 kDa. It plays an important role in the regulation of genes required for mitochondrial DNA transcription and replication. Researchers find NRF1 expression in various tissues including muscle brain and liver. This protein functions mechanistically by binding to specific promoter regions of target genes to control their expression making it essential for maintaining cellular energy metabolism.

Biological function summary

NRF1 influences the expression of genes involved in the mitochondrial function and is part of the transcriptional complex. This complex includes the NRF2 protein where NRF1 partners with co-activators to modulate gene transcription. NRF1 promotes the expression of components essential for mitochondrial biogenesis and cellular respiration which are vital processes for energy production in cells. Therefore NRF1 contributes to maintaining cellular energy homeostasis and overall cellular health.

Pathways

NRF1 integrates into critical regulatory pathways for mitochondrial function and metabolism. NRF1 links closely to the PGC-1α pathway which is instrumental in the regulation of energy metabolism and mitochondrial biogenesis. Additionally NRF1 works in conjunction with NRF2 to regulate oxidative stress responses. These interactions ensure a coordinated cellular response to energy demands and help protect the cell from oxidative damage.

NRF1 associates with neurodegenerative diseases and metabolic syndromes. Altered NRF1 regulation can impact diseases such as Alzheimer's disease where it may contribute to neurodegeneration through impaired mitochondrial function. Moreover the dysfunction of NRF1 might relate to metabolic disorders like diabetes as energy metabolism and mitochondrial health are disrupted. NRF1 connects with proteins like SIRT1 in these contexts further implicating NRF1 in these pathological states through its role in energy homeostasis and stress response.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

See full target information NRF1

대체 명칭 보기

Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

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제품이 사용된 논문 (55)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Investigational new drugs 43:669-678 PubMed40404898

2025

Peptide YY fragment PYY1-36 disrupts mitochondrial biogenesis via RBM43-dependent PGC-1α translation inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Benkun Liu,Fucheng Zhou,Bowen Shi,Yubo Yan,Yanbo Wang,Junfeng Wang,Yaoguo Lang,Shidong Xu

EMBO reports 26:1315-1343 PubMed39875582

2025

Mutant p53 regulates a distinct gene set by a mode of genome occupancy that is shared with wild type.

Applications

Unspecified application

Species

Unspecified reactive species

Ramy Rahmé,Lois Resnick-Silverman,Vincent Anguiano,Moray J Campbell,Pierre Fenaux,James J Manfredi

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2407574 PubMed39835574

2025

Extracellular Mitochondrial-Derived Vesicles Affect the Progression of Diabetic Foot Ulcer by Regulating Oxidative Stress and Mitochondrial Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Zhang,Zi Yan,Junyou Zhu,Ziyue Li,Lianglong Chen,Weihan Zheng,Zhenning Dai,Jiaxin Yang,Xinyi Yun,Yilin Wang,Hai Zhou,Ziwei Jiang,Qiuyi Yu,Shiyu Li,Wenhua Huang,Lei Yang

American journal of cancer research 14:4717-4730 PubMed39553210

2024

Knockdown of EPS8 expression attenuates the proliferation of enzalutamide-resistant prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Lun Huang,Sih-Han Chen,Richard Chen-Yu Wu,Hsing-Cha Mai,Chun-Hsien Wu,Pei-Fang Hsieh,See-Tong Pang,Victor Chia-Hsiang Lin

Frontiers in cell and developmental biology 12:1464773 PubMed39512900

2024

The impact of cannabinoid receptor 1 absence on mouse liver mitochondria homeostasis: insight into mitochondrial unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Rosalba Senese,Giuseppe Petito,Elena Silvestri,Maria Ventriglia,Nicola Mosca,Nicoletta Potenza,Aniello Russo,Sara Falvo,Francesco Manfrevola,Gilda Cobellis,Teresa Chioccarelli,Veronica Porreca,Vincenza Grazia Mele,Rosanna Chianese,Pieter de Lange,Giulia Ricci,Federica Cioffi,Antonia Lanni

BMC gastroenterology 24:97 PubMed38438958

2024

Nuclear respiratory factor 1 regulates super enhancer-controlled SPIDR to protect hepatocellular carcinoma cells from oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Baowang Liu,Jian Dou,Jinglin Cao

Science advances 9:eadi2767 PubMed37624892

2023

ANT-dependent MPTP underlies necrotic myofiber death in muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Bround,Julian R Havens,Allen J York,Michelle A Sargent,Jason Karch,Jeffery D Molkentin

Lipids in health and disease 22:33 PubMed36882837

2023

Exercise activates Sirt1-mediated Drp1 acetylation and inhibits hepatocyte apoptosis to improve nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zongqiang Hu,Hongyu Zhang,Yiting Wang,Boyi Li,Kaiyu Liu,Jianghua Ran,Li Li

Frontiers in aging neuroscience 14:1030807 PubMed36711211

2023

Identification and evaluation of midbrain specific longevity-related genes in exceptionally long-lived but healthy mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hyojung Kim,Yu-Jin Huh,Ji Hun Kim,Minkyung Jo,Joo-Heon Shin,Sang Chul Park,Jee-Yin Ahn,Yun-Il Lee,Yunjong Lee

Cancer genomics & proteomics 19:614-623 PubMed35985685

2022

Nuclear Respiratory Factor 1 Overexpression Inhibits Proliferation and Migration of PC3 Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Hsien Wu,Pei-Fang Hsieh,Yen-Hsi Lee,Wade Wei-Ting Kuo,Richard Chen-Yu Wu,Yung-Yao Lin,Chih-Hsin Hung,Ming-Lin Hsieh,See-Tong Pang,Yu-Lin Yang,Victor C Lin

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NRF1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody (AB34682)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody (AB34682)

Western blot - Anti-NRF1 antibody (AB34682)

Western blot - Anti-NRF1 antibody (AB34682)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (55)

Product promise