AB243913

Anti-nSMase antibody [EPR6718] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal nSMase antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

대체 명칭 보기

Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase, Lyso-PAF-PLC, N-SMase, nSMase, nSMase1, SMPD2

2 이미지

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Immunoprecipitation - Anti-nSMase antibody [EPR6718] - BSA and Azide free (AB243913)

ab131330 (purified) at 1 : 30 dilution (2μg) immunoprecipitating nSMase in Mouse brain lysate.
Lane 1 (input) : Mouse brain lysate 10μg
Lane 2 (+) : ab131330 & Mouse brain lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab131330 in Mouse brain lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

All lanes:

Immunoprecipitation - Anti-nSMase antibody [EPR6718] (<a href='/ko/products/primary-antibodies/nsmase-antibody-epr6718-ab131330'>ab131330</a>)

Predicted band size: 48 kDa

false

OI-RD Scanning - Anti-nSMase antibody [EPR6718] - BSA and Azide free (AB243913)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-nSMase antibody [EPR6718] - BSA and Azide free (AB243913)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6718

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab243913 is the carrier-free version of ab131330.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NSMase also known as neutral sphingomyelinase is an enzyme with a molecular mass typically ranging between 43 to 48 kDa. It plays an important role in the hydrolysis of sphingomyelin to generate ceramide and phosphocholine. nSMase is distributed widely in various tissues with higher expression levels observed in the brain liver and spleen. This enzyme exists in multiple isoforms each localized in different cellular compartments contributing to diverse cellular functions.

Biological function summary

Neutral sphingomyelinase influences several cellular processes by modulating ceramide levels. Ceramide a bioactive lipid serves as a secondary messenger in signal transduction pathways and mediates processes like apoptosis and cell differentiation. nSMase often functions as a monomer but it can also be part of larger complexes with other proteins to execute its roles in cellular signaling. Through its enzymatic activity nSMase impacts membrane structure and dynamics affecting overall cellular homeostasis.

Pathways

Neutral sphingomyelinase is involved in key signaling pathways like the MAPK and apoptosis pathways. In the MAPK pathway nSMase-derived ceramide acts as a signaling molecule that can activate kinases involved in stress responses. Additionally its role in apoptosis links it to various stress signals where it facilitates the programmed cell death process. Interaction with proteins such as caspase and protein kinase C highlights its integration into signaling networks illustrating its regulatory significance.

Altered nSMase activity associates with cancers and neurodegenerative diseases. In cancer changes in ceramide production by nSMase can impact cell proliferation and survival connecting it with oncogenic processes. In neurodegenerative disorders such as Alzheimer's disease dysregulated nSMase activity influences neuroinflammation and neuronal cell death. It's interaction with amyloid precursor protein in such conditions highlights the enzyme's potential as a therapeutic target for modulating disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes, at least in vitro, the hydrolysis of sphingomyelin to form ceramide and phosphocholine (PubMed : 10608884). Also hydrolyzes 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor) in vivo (PubMed : 10608884). Also acts on 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and sphingosylphosphocholine (PubMed : 10608884, PubMed : 14741383).

See full target information Sphingomyelin phosphodiesterase 2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death and differentiation : PubMed40770563

2025

ABCC10-mediated cGAMP efflux drives cancer cell radiotherapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyang Zhang,Jie Gao,Xiang Liao,Zining Zhang,Xiongfeng Cao,Yi Gong,Wenlong Chen,Lirong Zhang,Hsiang-I Tsai,Dongqing Wang,Haitao Zhu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-nSMase antibody [EPR6718] - BSA and Azide free

Immunoprecipitation - Anti-nSMase antibody [EPR6718] - BSA and Azide free (AB243913)

OI-RD Scanning - Anti-nSMase antibody [EPR6718] - BSA and Azide free (AB243913)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise