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AB214227

Anti-NSUN6 antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal NSUN6 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NSUN6 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

NOPD1, NSUN6, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN6 antibody (AB214227)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN6 antibody (AB214227)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling NSUN6 with ab214227 at 1/500 dilution, followed by secondary antibody detection and DAB staining.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human NSUN6 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8TEA1

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보완 제품

Primary Antibodies

AB272616

Anti-NSUN3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN3 antibody (AB272616)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB259941

Anti-NSUN2/SAKI antibody [EPR24140-93]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-NSUN2/SAKI antibody [EPR24140-93] (AB259941)

  • 0
  • 0
Primary Antibodies

AB271075

Anti-NOP2 antibody [EPR24888-92]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP2 antibody [EPR24888-92] (AB271075)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NSUN6 also known as NOP2/Sun RNA methyltransferase family member 6 is an RNA-modifying enzyme. It belongs to the NOL1/NOP2/Sun family and is responsible for catalyzing 5-methylcytosine formation on target RNA molecules playing an important role in RNA modification. The molecular mass of NSUN6 is approximately 56 kDa. It is expressed in various tissues but shows higher expression in the liver and skeletal muscle suggesting a potential specialization in these areas.
Biological function summary

NSUN6 contributes to the regulation of cellular processes by modifying RNA molecules. It is not currently identified as part of any known protein complex but significantly influences the stability and function of specific RNA targets. By this action NSUN6 can indirectly affect gene expression and downstream biological activities which might be critical during cellular growth and differentiation.

Pathways

NSUN6 interacts closely with pathways related to RNA processing and modification. It contributes to the epitranscriptomic landscape working alongside proteins such as NSUN2 another methyltransferase to maintain RNA modification balance. This activity impacts broader pathways like those regulating gene expression and protein synthesis exhibiting an interconnected role in fundamental cellular activities.

NSUN6 shows relevance to cancer and neurodevelopmental disorders. Alterations in its normal function could disrupt RNA methylation patterns leading to aberrant gene expression linked to these conditions. NSUN6's functional relationship with NSUN2 another RNA methyltransferase suggests that disruptions in their balance might contribute to these disorders highlighting their potential as targets for therapeutic intervention.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) (PubMed : 26160102, PubMed : 27703015, PubMed : 28531330). In vitro also methylates tRNA(Thr)(AGT) (PubMed : 26160102, PubMed : 27703015). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2 : 71 and 3 : 70) in the tRNA substrates (PubMed : 26160102, PubMed : 27703015).
See full target information NSUN6
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Medical principles and practice : international journal of the Kuwait University, Health Science Centre 33:56-65 PubMed38029727

2023

NSUN6 Regulates NM23-H1 Expression in an m5C Manner to Affect Epithelial-Mesenchymal Transition in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhansheng Lu,Bo Liu,Demiao Kong,Xiaojiang Zhou,Dengke Pei,Di Liu

Journal of cellular and molecular medicine 27:2398-2411 PubMed37408139

2023

RNA m5C methylation orchestrates BLCA progression via macrophage reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Dali Yan,Yongsong Xie,Liyuan Huang,Yi Zhang,Runhuan Gu,Huaibing Xie,Xing Huang,Hao Luo

Cancer management and research 13:7989-8002 PubMed34707405

2021

Comprehensive Analysis of the Prognostic Value and Immune Infiltrates of the Three-m5C Signature in Colon Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qishun Geng,Qian Wei,Zhibo Shen,Yuanyuan Zheng,Longhao Wang,Wenhua Xue,Lifeng Li,Jie Zhao

Scientific reports 7:5818 PubMed28724891

2017

Hypoxia-inducible factor-1 alpha is involved in RIP-induced necroptosis caused by in vitro and in vivo ischemic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Sa Yang,Tai-Long Yi,Sai Zhang,Zhong-Wei Xu,Ze-Qi Yu,Hong-Tao Sun,Cheng Yang,Yue Tu,Shi-Xiang Cheng
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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