AB236912

Anti-NTH1 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal NTH1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NTHL1.

대체 명칭 보기

NTH1, OCTS3, NTHL1, Endonuclease III-like protein 1, hNTH1, Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase, DNA glycosylase/AP lyase

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody (AB236912)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody (AB236912)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NTH1 using ab236912 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody (AB236912)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody (AB236912)

Paraffin-embedded human glioma tissue stained for NTH1 using ab236912 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NTHL1.

P78549

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The NTH1 protein also known as endonuclease III-like protein 1 functions as a DNA glycosylase involved in base excision repair. It plays a role in the repair of oxidative DNA damage. NTH1 has a molecular mass of approximately 33 kDa. This protein is located in various tissues throughout the human body but it shows higher expression in the testes heart and skeletal muscle. These expression patterns suggest its significance in oxidative stress response across different body systems.

Biological function summary

NTH1 removes oxidized pyrimidines from damaged DNA as part of its role in cellular repair mechanisms. As a component of the base excision repair pathway it tackles oxidative lesions to prevent mutation accumulation. It does not act as a complex with other proteins but operates after being recruited to relevant DNA sites requiring repair. This essential function keeps genomic integrity intact and allows for regular cellular processes.

Pathways

NTH1 plays an important role within the base excision repair and DNA damage response pathways. It cooperates with proteins such as APE1 which prepares the site for subsequent repair steps ensuring proper DNA maintenance. NTH1's involvement in base excision repair specifically addresses small-scale oxidative damage highlighting its role in safeguarding genetic information. These pathways are fundamental for normal cell function and response to environmental stressors.

NTH1 has links to conditions involving accumulated DNA damage such as cancer and neurodegenerative diseases. DNA repair deficiency due to NTH1 malfunction can contribute to the onset of such diseases. The protein interacts with partners like OGG1 which also plays a role in oxidative DNA lesion recognition and repair to manage DNA stability. Understanding the dysfunction of NTH1 in these contexts helps in exploring potential therapeutic targets and diagnostic markers for related disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage (PubMed : 29610152, PubMed : 9927729). The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Also has 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.

See full target information NTHL1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 12:19936 PubMed36402786

2022

Complementary anti-cancer pathways triggered by inhibition of sideroflexin 4 in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lia Tesfay,Bibbin T Paul,Poornima Hegde,Molly Brewer,Samrin Habbani,Evan Jellison,Timothy Moore,Hao Wu,Suzy V Torti,Frank M Torti

Experimental and therapeutic medicine 24:614 PubMed36160886

2022

TMEM14A aggravates the progression of human ovarian cancer cells by enhancing the activity of glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingmei Zhang,Xiaohong Wang,Xuan Zhang,Jingfen Zhan,Binbin Zhang,Jin Jia,Jie Chen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NTH1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody (AB236912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody (AB236912)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise