AB171741

Anti-NUBPL antibody [EPR11833]

RabMAb
Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal NUBPL antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

대체 명칭 보기

C14orf127, NUBPL, Iron-sulfur cluster transfer protein NUBPL, IND1 homolog, Nucleotide-binding protein-like, huInd1

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-NUBPL antibody [EPR11833] (AB171741)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUBPL antibody [EPR11833] (AB171741)

Immunofluorescent analysis of HepG2 cells labeling NUBPL using ab171741 at 1/50 dilution.

Supplier Data

Immunoprecipitation - Anti-NUBPL antibody [EPR11833] (AB171741)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling NUBPL, or 1X PBS (Lane 2 : negative control) using ab171741 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-NUBPL antibody [EPR11833] (ab171741)

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-NUBPL antibody [EPR11833] (AB171741)

All lanes:

Western blot - Anti-NUBPL antibody [EPR11833] (ab171741) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Predicted band size: 34 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11833

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NUBPL also known as nucleotide-binding protein-like and IND1 is a mitochondrial matrix protein with a molecular mass of approximately 34 kDa. It is expressed in various tissues being notably present in high-energy demand tissues like heart and skeletal muscle. Mechanically NUBPL plays a critical role in the assembly of complex I of the mitochondrial respiratory chain by aiding the incorporation of Fe-S clusters which are essential for catalytic activity.

Biological function summary

NUBPL functions as a chaperone-like protein ensuring proper assembly of complex I which is part of the mitochondrial respiratory chain. NUBPL operates as a component of the mitochondrial iron-sulfur (Fe-S) cluster assembly complex. It directly interacts with Fe-S protein synthesis an essential part of cellular respiration. The precise regulation of this complex facilitates energy production within cells by supporting efficient electron transport.

Pathways

NUBPL is integral to the mitochondrial oxidative phosphorylation pathway a process that generates ATP the main energy currency of cells. It associates with the iron-sulfur (Fe-S) cluster biogenesis pathway working alongside proteins such as ISCU and NFS1. These interactions ensure the effective transfer and stabilization of Fe-S clusters necessary for mitochondrial function and metabolic processes.

NUBPL mutations have been linked to mitochondrial complex I deficiency which can lead to a spectrum of neurodegenerative conditions including Leigh syndrome. The disease often involves interactions with proteins such as NDUFS4 another complex I assembly factor. Additionally possible links may exist between NUBPL dysfunction and mitochondrial diseases characterized by impaired energy production and accumulation of metabolic intermediates highlighting the protein's importance in maintaining cellular energy homeostasis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Iron-sulfur cluster transfer protein involved in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) (PubMed : 19752196). May deliver one or more Fe-S clusters to complex I subunits (PubMed : 19752196).

See full target information NUBPL

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular therapy. Nucleic acids 35:102090 PubMed38187140

2024

Altered CELF4 splicing factor enhances pancreatic neuroendocrine tumors aggressiveness influencing mTOR and everolimus response.

Applications

Unspecified application

Species

Unspecified reactive species

Emilia Alors-Pérez,Sergio Pedraza-Arevalo,Ricardo Blázquez-Encinas,Víctor García-Vioque,Antonio Agraz-Doblas,Elena M Yubero-Serrano,Marina E Sánchez-Frías,Raquel Serrano-Blanch,María Ángeles Gálvez-Moreno,Francisco Gracia-Navarro,Manuel D Gahete,Álvaro Arjona-Sánchez,Raúl M Luque,Alejandro Ibáñez-Costa,Justo P Castaño

Aging cell 22:e13954 PubMed37614052

2023

Alterations of lipid-mediated mitophagy result in aging-dependent sensorimotor defects.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Oleinik,Onder Albayram,Mohamed Faisal Kassir,F Cansu Atilgan,Chase Walton,Eda Karakaya,John Kurtz,Alexander Alekseyenko,Habeeb Alsudani,Megan Sheridan,Zdzislaw M Szulc,Besim Ogretmen

Orphanet journal of rare diseases 17:386 PubMed36280881

2022

Early embryonic lethality in complex I associated p.L104P Nubpl mutant mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Cheng,James Cleak,Lan Weiss,Heather Cater,Michelle Stewart,Sara Wells,Rod Carlo Columbres,Alyaa Shmara,C Alejandra Morato Torres,Faria Zafar,Birgitt Schüle,Jonathan Neumann,Eli Hatchwell,Virginia Kimonis

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUBPL antibody [EPR11833]

Immunocytochemistry/ Immunofluorescence - Anti-NUBPL antibody [EPR11833] (AB171741)

Immunoprecipitation - Anti-NUBPL antibody [EPR11833] (AB171741)

Western blot - Anti-NUBPL antibody [EPR11833] (AB171741)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Sample Prep & Detection Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise