AB50279

Anti-Nucleolin antibody

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(40 제품이 사용된 논문 )

Rabbit Polyclonal Nucleolin antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 40 publications. Immunogen corresponding to Synthetic Peptide within Human NCL aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

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Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody (AB50279)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody (AB50279)

Indirect Immunofluorescence Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 µg/mL Anti-Nucleolin (ab50279). The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, Flow Cyt, IP, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NCL aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P19338

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "1-2 ug/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10-20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "1-2 ug/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "1-2 ug/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

This antibody has been affinity-purified using the immunizing peptideimmobilized on agarose.

보관 버퍼

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

Biological function summary

The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

Pathways

The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

See full target information NCL

대체 명칭 보기

Nucleolin, Protein C23, NCL

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제품이 사용된 논문 (40)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 53: PubMed40613709

2025

Depletion of BRD9-mediated R-loop accumulation inhibits leukemia cell growth via transcription-replication conflict.

Applications

Unspecified application

Species

Unspecified reactive species

Seo Yun Lee,Soo Hyeon Lee,Min Jae Kwak,Ja Young Kim,Jullian O Perren,Kyle M Miller,Jae Jin Kim

The Journal of biological chemistry 300:107996 PubMed39547511

2024

The molecular chaperone ALYREF promotes R-loop resolution and maintains genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Jay Bhandari,Cristina Guillén-Mendoza,Kathryn Banks,Lillian Eliaz,Sierra Southwell,Darriel Eyaa,Rosa Luna,Andrés Aguilera,Xiaoyu Xue

Nucleic acids research 52:8643-8660 PubMed38936834

2024

PCAF promotes R-loop resolution via histone acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Seo Yun Lee,Soo Hyeon Lee,Nak Hun Choi,Ja Young Kim,Jun Hee Kweon,Kyle M Miller,Jae Jin Kim

RNA (New York, N.Y.) 30:839-853 PubMed38609156

2024

Cytosolic RNA binding of the mitochondrial TCA cycle enzyme malate dehydrogenase.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle Noble,Aindrila Chatterjee,Thileepan Sekaran,Thomas Schwarzl,Matthias W Hentze

Molecular cell 84:1475-1495.e18 PubMed38521065

2024

Transcription elongation defects link oncogenic SF3B1 mutations to targetable alterations in chromatin landscape.

Applications

Unspecified application

Species

Unspecified reactive species

Prajwal C Boddu,Abhishek K Gupta,Rahul Roy,Bárbara De La Peña Avalos,Anne Olazabal-Herrero,Nils Neuenkirchen,Joshua T Zimmer,Namrata S Chandhok,Darren King,Yasuhito Nannya,Seishi Ogawa,Haifan Lin,Matthew D Simon,Eloise Dray,Gary M Kupfer,Amit Verma,Karla M Neugebauer,Manoj M Pillai

Cell reports 43:113610 PubMed38165804

2024

The FANCI/FANCD2 complex links DNA damage response to R-loop regulation through SRSF1-mediated mRNA export.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Olazabal-Herrero,Boxue He,Youngho Kwon,Abhishek K Gupta,Arijit Dutta,Yuxin Huang,Prajwal Boddu,Zhuobin Liang,Fengshan Liang,Yaqun Teng,Li Lan,Xiaoyong Chen,Huadong Pei,Manoj M Pillai,Patrick Sung,Gary M Kupfer

Nature communications 14:6890 PubMed37898641

2023

The chromatin network helps prevent cancer-associated mutagenesis at transcription-replication conflicts.

Applications

Unspecified application

Species

Unspecified reactive species

Aleix Bayona-Feliu,Emilia Herrera-Moyano,Nibal Badra-Fajardo,Iván Galván-Femenía,María Eugenia Soler-Oliva,Andrés Aguilera

Molecular cell 83:3707-3719.e5 PubMed37827159

2023

DICER ribonuclease removes harmful R-loops.

Applications

Unspecified application

Species

Unspecified reactive species

Lola P Camino,Arijit Dutta,Sonia Barroso,Carmen Pérez-Calero,Jeffrey N Katz,María García-Rubio,Patrick Sung,Belén Gómez-González,Andrés Aguilera

Science advances 9:eadi0286 PubMed37506203

2023

PTBP1 regulates injury responses and sensory pathways in adult peripheral neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Alber,Pierluigi Di Matteo,Matthew D Zdradzinski,Irene Dalla Costa,Katalin F Medzihradszky,Riki Kawaguchi,Agostina Di Pizio,Philip Freund,Nicolas Panayotis,Letizia Marvaldi,Ella Doron-Mandel,Nataliya Okladnikov,Ida Rishal,Reinat Nevo,Giovanni Coppola,Seung Joon Lee,Pabitra K Sahoo,Alma L Burlingame,Jeffery L Twiss,Mike Fainzilber

Nature communications 14:2074 PubMed37045843

2023

The RNA-binding protein landscapes differ between mammalian organs and cultured cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joel I Perez-Perri,Dunja Ferring-Appel,Ina Huppertz,Thomas Schwarzl,Sudeep Sahadevan,Frank Stein,Mandy Rettel,Bruno Galy,Matthias W Hentze

제품이 사용된 논문 모두 보기

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Product promise

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Anti-Nucleolin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody (AB50279)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (40)

Product promise