AB155977

Anti-Nucleolin (phospho T84) antibody [EPR8080]

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(4 제품이 사용된 논문 )

Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977) is a rabbit monoclonal antibody detecting Nucleolin in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (AB155977)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (AB155977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Nucleolin with purified ab155977 at 1/10000 dilution (0.18 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) (ready to use) secondary antibody was used.

Nuclear staining on human tonsil without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

The section was incubated with ab155977 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (AB155977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcioma tissue sections labeling Nucleolin with purified ab155977 at 1/10000 dilution (0.18 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) (ready to use) secondary antibody was used.

Nuclear staining on human kidney carcinoma without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

The section was incubated with ab155977 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Unknown

Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (AB155977)

All lanes:

Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977) at 1/5000 dilution

Lane 1:

Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate at 15 µg

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast) treated with 100nM Calyculin A for 30 minutes, then the membrane treated with Lambda Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 76 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8080

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "For unpurified use at 1/50 - 1/100 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

제품 세부 정보

What is this antibody validated in?
Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Nucleolin?
Anti-Nucleolin (phospho T84) [EPR8080] (ab155977) specifically detects a band for Nucleolin (UniProt: P19338) at a molecular weight of 77kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR8080] also available for your convenience: ab155977, Carrier free - ab233474, PE - ab317906, APC - ab317945, Alexa Fluor® 488 - ab317986, Alexa Fluor® 594 - ab318028, Alexa Fluor® 555 - ab318070, Alexa Fluor® 647 - ab318111, Alexa Fluor® 750 - ab321142

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

Biological function summary

The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

Pathways

The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

See full target information NCL pT84

대체 명칭 보기

Nucleolin, Protein C23, NCL

style

left-column

제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록and refine your search

Nature 646:734-745 PubMed40836083

2025

Targeting G1-S-checkpoint-compromised cancers with cyclin A/B RxL inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Shilpa Singh,Catherine E Gleason,Min Fang,Yasmin N Laimon,Vishal Khivansara,Shanhai Xie,Yavuz T Durmaz,Aniruddha Sarkar,Kenneth Ngo,Varunika Savla,Yixiang Li,Muhannad Abu-Remaileh,Xinyue Li,Marie-Anais Locquet,Bishma Tuladhar,Ranya Odeh,Frances Hamkins-Indik,Daphne He,Miles W Membreno,Meisam Nosrati,Nathan N Gushwa,Siegfried S F Leung,Breena Fraga-Walton,Luis Hernandez,Miguel P Baldomero,Bryan M Lent,David Spellmeyer,Joshua F Luna,Dalena Hoang,Yuliana Gritsenko,Manesh Chand,Megan K DeMart,Sammy Metobo,Chinmay Bhatt,Justin A Shapiro,Kai Yang,Nathan J Dupper,Andrew T Bockus,Jinshu Fang,Ramesh Bambal,Peadar Cremin,John G Doench,James B Aggen,Li-Fen Liu,Bernard Levin,Evelyn W Wang,Iolanda Vendrell,Roman Fischer,Benedikt Kessler,Prafulla C Gokhale,Sabina Signoretti,Alexander Spektor,Constantine Kreatsoulas,Marie Evangelista,Rajinder Singh,David J Earp,Deepak Nijhawan,Pablo D Garcia,Matthew G Oser

NPJ breast cancer 11:39 PubMed40287441

2025

MUC1-C dependency in drug resistant HR+/HER2- breast cancer identifies a new target for antibody-drug conjugate treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Ayako Nakashoji,Atrayee Bhattacharya,Hiroki Ozawa,Naoki Haratake,Keisuke Shigeta,Atsushi Fushimi,Nami Yamashita,Akira Matsui,Shoko Kure,Tomoe Kameyama,Makoto Takeuchi,Kazumasa Fukuda,Takamichi Yokoe,Aiko Nagayama,Tetsu Hayahsida,Yuko Kitagawa,Renyan Liu,Antonio Giordano,Rinath Jeselsohn,Geoffrey I Shapiro,Donald Kufe

Cancer discovery 14:446-467 PubMed38047585

2023

INX-315, a Selective CDK2 Inhibitor, Induces Cell Cycle Arrest and Senescence in Solid Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Dietrich,Alec Trub,Antonio Ahn,Michael Taylor,Krutika Ambani,Keefe T Chan,Kun-Hui Lu,Christabella A Mahendra,Catherine Blyth,Rhiannon Coulson,Susanne Ramm,April C Watt,Sunil Kumar Matsa,John Bisi,Jay Strum,Patrick Roberts,Shom Goel

Nature communications 13:2835 PubMed35595767

2022

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Zhang,Yichao Gan,Hongzhi Li,Jie Yin,Xin He,Liming Lin,Senlin Xu,Zhipeng Fang,Byung-Wook Kim,Lina Gao,Lili Ding,Eryun Zhang,Xiaoxiao Ma,Junfeng Li,Ling Li,Yang Xu,David Horne,Rongzhen Xu,Hua Yu,Ying Gu,Wendong Huang

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleolin (phospho T84) antibody [EPR8080]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (AB155977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (AB155977)

Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (AB155977)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise