Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11]

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11] (AB324964)

Indirect ELISA analysis of ab324964 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Glycoprotein N Crimean-Congo hemorrhagic fever virus, Nucleoprotein Crimean-Congo hemorrhagic fever virus.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11] (AB324964)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11] (ab324964) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Glycoprotein N Crimean-Coo hemorrhagic fever virus expression vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Nucleoprotein Crimean-Coo hemorrhagic fever virus expression vector containing a myc-his-tag whole cell lysate at 1 µg

Lane 4:

293T cells transfected with a Glycoprotein C Crimean-Coo hemorrhagic fever virus expression vector containing a myc-his-tag whole cell lysate at 4 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 53 kDa,36 kDa

false

Exposure time: 4s