AB221994

Anti-NUDT21/CFIM25 antibody

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Rabbit Polyclonal NUDT21/CFIM25 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NUDT21 aa 1-150.

대체 명칭 보기

CFIM25, CPSF25, CPSF5, NUDT21, Cleavage and polyadenylation specificity factor subunit 5, Cleavage and polyadenylation specificity factor 25 kDa subunit, Cleavage factor Im complex 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix hydrolase 21, Pre-mRNA cleavage factor Im 68 kDa subunit, CPSF 25 kDa subunit, CFIm25, Nudix motif 21

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-NUDT21/CFIM25 antibody (AB221994)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUDT21/CFIM25 antibody (AB221994)

PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for NUDT21/CFIM25 (green) using ab221994 at 4 μg/ml in ICC/IF.

Supplier Data

Western blot - Anti-NUDT21/CFIM25 antibody (AB221994)

All lanes:

Western blot - Anti-NUDT21/CFIM25 antibody (ab221994) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 26 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NUDT21 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O43809

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NUDT21 also known as CFIm25 is a protein important for RNA processing. This protein is part of the cleavage factor Im complex and plays an important role in pre-mRNA polyadenylation. NUDT21 is about 25 kDa in molecular weight and expresses widely across human tissues. It is especially abundant in the brain and liver indicating important roles in these areas.

Biological function summary

NUDT21 acts as a component of the cleavage factor I which is essential for mRNA polyadenylation. This protein ensures proper 3’-end processing of pre-mRNA by interacting with other CFIm complex components like CPSF and CstF. This interaction is necessary for the regulation of mRNA stability and eventual translation into proteins. The correct functioning of NUDT21 is necessary for the maintenance of gene expression.

Pathways

NUDT21 is part of the mRNA 3'-end processing pathway. This pathway is critical for proper gene expression affecting mRNA synthesis and degradation rates. Within this pathway NUDT21 works closely with proteins such as CPSF6 and CPSF7 facilitating the assembly of the mRNA cleavage complex. The pathway influences general RNA growth and stability impacting numerous cellular processes.

Abnormalities in NUDT21 are linked to neurological disorders and cancer. Loss of function or altered expression is connected to intellectual disability affecting neural development and cognitive abilities. In the context of cancer NUDT21 levels can influence tumor growth and progression. Changes in NUDT21 may cause dysregulation in associated proteins like CPSF6 further complicating these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed : 14690600, PubMed : 15937220, PubMed : 17024186, PubMed : 17098938, PubMed : 29276085, PubMed : 8626397, PubMed : 9659921). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed : 14690600, PubMed : 17024186, PubMed : 8626397, PubMed : 9659921). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed : 17098938, PubMed : 23187700, PubMed : 29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed : 17098938, PubMed : 20695905, PubMed : 29276085). NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7 (PubMed : 29276085). Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing (PubMed : 14690600, PubMed : 15169763, PubMed : 17024186, PubMed : 20479262, PubMed : 22813749, PubMed : 8626397). The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA (PubMed : 20479262, PubMed : 21295486). Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function (By similarity). Binds to chromatin (By similarity). Binds to, but does not hydrolyze mono- and di-adenosine nucleotides (PubMed : 18445629).

See full target information NUDT21

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Diabetology & metabolic syndrome 13:152 PubMed34963484

2021

MiR-423-5p activated by E2F1 promotes neovascularization in diabetic retinopathy by targeting HIPK2.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Xiao,Yinu Zhao,Hongjing Sun,Jia Xu,Wenjie Li,Limo Gao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUDT21/CFIM25 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NUDT21/CFIM25 antibody (AB221994)

Western blot - Anti-NUDT21/CFIM25 antibody (AB221994)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise