Anti-NuMA antibody [EP3976] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] - BSA and Azide free (AB239963)

ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109262).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody [EP3976] - BSA and Azide free (AB239963)

ab109262, at 1/100 dilution, staining NuMA in HeLa cells by Immunofluorescence.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109262).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] - BSA and Azide free (AB239963)

ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast carcinoma by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109262).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] - BSA and Azide free (AB239963)

This data was developed using ab109262, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling NuMA with ab109262 at 1/5000 dilution.

Nuclear staining on mouse testis.

The section was incubated with ab109262 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] - BSA and Azide free (AB239963)

This data was developed using ab109262, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse pancreatic cancer tissue labeling NuMA with ab109262 at 1/5000 dilution.

Nuclear staining on mouse pancreatic cancer.

The section was incubated with ab109262 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] - BSA and Azide free (AB239963)

This data was developed using ab109262, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NuMA with ab109262 at 1/5000 dilution.

Nuclear staining on mouse kidney.

The section was incubated with ab109262 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody [EP3976] - BSA and Azide free (AB239963)

This data was developed using ab109262, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) labelling NuMA with ab109262 at 1/500 (4.12 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody as secondary at 1/1000 (2 μg/ml) dilution. Confocal image showing nuclear and weak cytoplasmic staining in NIH/3T3 cell line (shown in green). ab7291 Anti-alpha Tubulin mouse monoclonal antibody at 1/1000 (1μg/ml) was used as a counterstain along with ab150120 as secondary at 1/1000 (2μg/ml) (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• WB

Lab

Western blot - Anti-NuMA antibody [EP3976] - BSA and Azide free (AB239963)

This data was developed using ab109262, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] - Loading Control (ab129002) staining at 1/5000 dilution (124KDa).

All lanes:

Western blot - Anti-NuMA antibody [EP3976] (<a href='/ko/products/primary-antibodies/numa-antibody-ep3976-ab109262'>ab109262</a>) at 1/5000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 10 µg

Lane 2:

NIH/3T3 transfected with siRNA specifically targeting NuMA whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 238 kDa,124 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-NuMA antibody [EP3976] - BSA and Azide free (AB239963)

This data was developed using ab109262, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] - Loading Control (ab129002) staining at 1/5000 dilution (124KDa).

The identity of the lower MW bands at approximately 100 + 120 kDa (in lanes 1-4) are unknown.

All lanes:

Western blot - Anti-NuMA antibody [EP3976] (<a href='/ko/products/primary-antibodies/numa-antibody-ep3976-ab109262'>ab109262</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 238 kDa,124 kDa

false

Exposure time: 37s