AB236634

Anti-Nup107 antibody [EPR22877-21]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Nup107 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

대체 명칭 보기

Nuclear pore complex protein Nup107, 107 kDa nucleoporin, Nucleoporin Nup107, NUP107

3 이미지

Unknown

Immunoprecipitation - Anti-Nup107 antibody [EPR22877-21] (AB236634)

Nup107 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10ug with ab236634 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236634. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10ug

Lane 2 : ab236634 IP in HEK-293T whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab236634 in HEK-293T whole cell lysate.

Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

Exposure time : 6 seconds

All lanes:

Immunoprecipitation - Anti-Nup107 antibody [EPR22877-21] (ab236634)

Predicted band size: 106 kDa,90 kDa

Observed band size: 107 kDa,97 kDa

false

Lab

Western blot - Anti-Nup107 antibody [EPR22877-21] (AB236634)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times :

Lanes 1-2 : 3 minutes; Lane 3 : 15 seconds.

All lanes:

Western blot - Anti-Nup107 antibody [EPR22877-21] (ab236634) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 106 kDa

Observed band size: 107 kDa

false

Lab

Western blot - Anti-Nup107 antibody [EPR22877-21] (AB236634)

This antibody reacts with an unidentifiable protein around 37 kDa.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Nup107 antibody [EPR22877-21] (ab236634) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 106 kDa

Observed band size: 107 kDa

false

Exposure time: 15s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22877-21

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Nup107 also known as nucleoporin 107 is an important protein component of the nuclear pore complex (NPC). With a molecular mass of approximately 107 kDa it is a significant element in the structure of the NPC. Nup107 is expressed in various tissues throughout the body. It plays an essential mechanical role in the assembly and maintenance of NPCs facilitating the transport of macromolecules between the nucleus and the cytoplasm.

Biological function summary

Nup107 forms part of the Nup107-160 subcomplex which contributes to the NPC's structural integrity and function. This complex acts as a scaffold providing support for the overall architecture and positioning within the nuclear envelope. The presence of Nup107 influences nuclear-cytoplasmic transport which is key in regulation of gene expression and cell cycle progression. Nup107 ensures proper assembly serving as an anchor for other nucleoporins within the NPC.

Pathways

The Nup107 subcomplex plays a critical role in key cellular processes such as the cell cycle and gene expression regulation. It participates in the mitotic entry and exit by regulating nucleocytoplasmic transport of cell cycle regulators and transcription factors. Nup107 interacts with proteins like Nup133 and Nup160 which assist in these processes. The precise functioning of Nup107 is necessary for maintaining cellular homeostasis and successful progression through cell division.

Nup107 has links to conditions such as primary ovarian insufficiency and nephrotic syndrome. Mutations or aberrant expression of Nup107 disrupt NPC assembly or function potentially leading to impaired cellular transport and altered cellular functions. In ovarian insufficiency altered Nup107 expression affects folliculogenesis by disrupting nuclear transport of specific factors. In nephrotic syndrome Nup107 dysfunction impacts the glomerular filtration barrier with associated proteins like nephrin and podocin demonstrating interrelated pathological effects.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance (PubMed : 12552102, PubMed : 15229283, PubMed : 30179222). Required for the assembly of peripheral proteins into the NPC (PubMed : 12552102, PubMed : 15229283). May anchor NUP62 to the NPC (PubMed : 15229283). Involved in nephrogenesis (PubMed : 30179222).

See full target information Nuclear pore complex protein Nup107

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nup107 antibody [EPR22877-21]

Immunoprecipitation - Anti-Nup107 antibody [EPR22877-21] (AB236634)

Western blot - Anti-Nup107 antibody [EPR22877-21] (AB236634)

Western blot - Anti-Nup107 antibody [EPR22877-21] (AB236634)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise