Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (AB236010)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NUP133 with purified ab155990 at 1 : 50 (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155990).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (AB236010)

Immunofluorescent analysis of HeLa cells labeling NUP133 with unpurified ab155990 at 1/50 dilution. This image was produced using unpurified antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155990).

• ICC/IF

AbReview45106****

Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (AB236010)

Unpurified ab155990 staining NUP133 in mokey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor^® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. This image was produced using unpurified antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155990).

This image is courtesy of an Abreview submitted by Aaron Halpen