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AB242202

Anti-Occludin antibody [CL1555]

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(1 리뷰)

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(9 제품이 사용된 논문 )

Mouse Monoclonal Occludin antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OCLN aa 250-450.

대체 명칭 보기

Occludin, OCLN

9 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)

Formalin-fixed, paraffin-embedded human colon tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Occludin with ab242202 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling Occludin with ab242202 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry - Anti-Occludin antibody [CL1555] (AB242202)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Occludin antibody [CL1555] (AB242202)

PFA-fixed, Triton X-100 permeabilized CACO-2 cells stained for OCLN (Green) using ab242202 at 4 μg/ml in ICC/IF.

Microtublues are stained red, nuclei are stained blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)

Formalin-fixed, paraffin-embedded human liver tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (AB242202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeeltal muscle tissue labelling Occludin with ab242202 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Occludin antibody [CL1555] (AB242202)
  • WB

Supplier Data

Western blot - Anti-Occludin antibody [CL1555] (AB242202)

All lanes:

Western blot - Anti-Occludin antibody [CL1555] (ab242202) at 1 µg/mL

Lane 1:

Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

Lane 2:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate

Predicted band size: 59 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL1555

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human OCLN aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q16625

Epitope

Binds to an epitope located within the peptide sequence TSPVDDFRQPRYSSG as determined by overlapping synthetic peptides.

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대체품

Primary Antibodies

AB216327

Anti-Occludin antibody [EPR20992]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (AB216327)

  • 4
  • 7
Primary Antibodies

AB224526

Anti-Occludin antibody [EPR20992] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526)

  • 4
  • 2
Primary Antibodies

AB222691

Anti-Occludin antibody

Flow Cytometry - Anti-Occludin antibody (AB222691)

  • 5
  • 1
Primary Antibodies

AB31721

Anti-Occludin antibody

Western blot - Anti-Occludin antibody (AB31721)

  • 4
  • 19

보완 제품

Primary Antibodies

AB221547

Anti-ZO1 tight junction protein antibody [EPR19945-296]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

  • 5
  • 6
Primary Antibodies

AB211737

Anti-Claudin 1 antibody [EPRR18871]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Claudin 1 antibody [EPRR18871] (AB211737)

  • 5
  • 7
Primary Antibodies

AB131259

Anti-Claudin 5 antibody [EPR7583]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Claudin 5 antibody [EPR7583] (AB131259)

  • 4
  • 7
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2-10 µg/mL", "ICC-species-notes": "<p>Fixation: PFA/Triton X-100.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
Purified from TCS.
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Occludin is a protein that plays an important mechanical role in forming tight junctions which are specialized connections between neighboring cell membranes. Alternative names for this target include occludin-1. The occludin protein has a molecular weight of approximately 65 kDa. It is expressed in a variety of tissues but levels tend to be high in epithelial and endothelial cells where tight junctions are essential for barrier functions. Occludin acts as integral membrane protein contributing to the complex structures that seal spaces between cells.
Biological function summary

Occludin serves as an important component in maintaining cell-to-cell adhesion within tight junction complexes. It interacts with other tight junction proteins such as claudins and junctional adhesion molecules to regulate paracellular permeability. These interactions help maintain selective barrier properties of epithelial and endothelial layers preventing the passage of large molecules and pathogens while allowing passage of ions and small molecules. Occludin stabilizes the tight junctions by interacting with the cytoskeleton and signaling proteins that are important for tight junction assembly and maintenance.

Pathways

Occludin functions prominently in the regulation of tight junction integrity and barrier function. It participates in pathways such as the tight junction signaling pathway and the paracellular transport pathway. Occludin interacts with ZO-1 a scaffolding protein bridging its interaction with the actin cytoskeleton which supports the structural organization and function of tight junctions. This connectivity allows for regulation of the permeability and transport across the epithelial barrier.

Disruptions in occludin function are connected to conditions such as inflammatory bowel disease (IBD) and some cancers. In IBD abnormal occludin expression can compromise intestinal barrier function leading to increased intestinal permeability and an inflammatory response. In certain cancers loss of occludin expression is linked to increased invasiveness and metastasis due to weakened cell adhesion. The dysfunction of proteins like ZO-1 which interact with occludin in these pathways also contributes to these pathological states highlighting the importance of occludin's regulatory roles in maintaining cellular barrier integrity.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
See full target information OCLN
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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Toxicological sciences : an official journal of the Society of Toxicology 201:48-60 PubMed38830033

2024

Developmental PFOS exposure alters lung inflammation and barrier integrity in juvenile mice.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph H Lucas,Qixin Wang,Jiries Meehan-Atrash,Cortney Pang,Irfan Rahman

Experimental and therapeutic medicine 26:319 PubMed37273752

2023

iRHOM2 regulates inflammation and endothelial barrier permeability via CX3CL1.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyuan Yan,Junsong Wu,Huilian Yan

Toxicological sciences : an official journal of the Society of Toxicology 190:215-226 PubMed36106993

2022

Perfluorooctane Sulfonic Acid Disrupts Protective Tight Junction Proteins via Protein Kinase D in Airway Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph H Lucas,Qixin Wang,Irfan Rahman

Investigative ophthalmology & visual science 63:25 PubMed35895036

2022

LGALS3BP in Microglia Promotes Retinal Angiogenesis Through PI3K/AKT Pathway During Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Zhao,Yusen Liu,Jiayu Meng,Xiaotang Wang,Xianyang Liu,Wanqian Li,Qian Zhou,Junjie Xiang,Na Li,Shengping Hou

Chemical research in toxicology 35:731-749 PubMed35405071

2022

Mycotoxins Activate the Aryl Hydrocarbon Receptor and Nrf2-ARE Pathway to Alter the Structure and Immune Response of Colon Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Groestlinger,Veronika Spindler,Gudrun Pahlke,Michael Rychlik,Giorgia Del Favero,Doris Marko

Bioengineered 13:5205-5215 PubMed35170371

2022

Oxycodone relieves permeability damage and apoptosis of oxygen-glucose deprivation/reoxygenation-induced brain microvascular endothelial cells through ras homolog family member A (RhoA)/ Rho-associated coiled-coil containing kinases (ROCK)/ myosin light chain 2 (MLC2) signal.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Shao,Dong Han,Yihui Shen,Wen Bian,Liting Zou,Yiqian Hu,Wei Sun

Oncology letters 23:84 PubMed35126726

2022

Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Mao,Jun Wang,Fen Luo

Bioengineered 12:4899-4910 PubMed34374627

2021

miR-152-3p aggravates vascular endothelial cell dysfunction by targeting DEAD-box helicase 6 (DDX6) under hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyan Zhao,Chanji Wu,Xiangying He,Eryi Zhao,Shijun Hu,Yeguang Han,Ting Wang,Yanquan Chen,Tao Liu,Shixiong Huang

Oncology reports 45:65-72 PubMed33416135

2021

Methylation of the miR‑29b‑3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Wang,Nv Mu,Nina Qu
제품이 사용된 논문 모두 보기
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