Rabbit Polyclonal Oct4 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU5F1 aa 1-200.
대체 명칭 보기
OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)
Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1 : 100 dilution. Lower image shows cells co-stained with DAPI.
- Flow Cyt
Unknown
Flow Cytometry - Anti-Oct4 antibody (AB137427)
Flow cytometric analysis of Human Embryonic Stem cells labelling Oct4 with ab137427 at 1 : 50 dilution (green) or rabbit IgG (black).
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)
Immunofluorescent analysis of paraformaldehyde-fixed Human Embryonic Stem cells labelling Oct4 with ab137427 at 1 : 400 dilution. Lower image shows cells co-stained with Hoechst 33342.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)
Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1 : 200 dilution.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)
Immunofluorescence analysis of NT2D1 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Oct4 protein using ab137427 at a 1/500 dilution (Green). phalloidin, a cytoskeleton marker was stained at a 1/200 dilution (Red). Scale bar= 10µm.
- WB
Supplier Data
Western blot - Anti-Oct4 antibody (AB137427)
Samples were separated by 10% SDS-PAGE.
All lanes:
Western blot - Anti-Oct4 antibody (ab137427) at 1/5000 dilution
All lanes:
Human ESC tissue extract at 30 µg
Secondary
All lanes:
HRP-conjugated anti-rabbit IgG
Predicted band size: 38 kDa
false
Reactivity 정보
특성 및 보관 정보
제형
Purification 테크닉
Purification 관련 사항
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.
Pathways
Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.
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타겟 정보
제품이 사용된 논문 (16)
Recent publications for all applications. Explore the 전체 목록 and refine your search
BioImpacts : BI 13:289-300 PubMed37645025
2023
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Analytical cellular pathology (Amsterdam) 2022:5632164 PubMed36247876
2022
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Frontiers in veterinary science 8:706106 PubMed34350230
2021
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Animals : an open access journal from MDPI 10: PubMed33066219
2020
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Oncology reports 44:577-588 PubMed32627006
2020
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Cell biochemistry and function 38:1069-1078 PubMed32390141
2020
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Oncology letters 20:794-802 PubMed32566006
2020
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Experimental & molecular medicine 51:1-20 PubMed31757938
2019
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Theriogenology 142:184-195 PubMed31604179
2019
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Cancer management and research 11:5075-5089 PubMed31213919
2019
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Product promise
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